LNCIB human full-length cDNAs collection

Towards a better comprehension of the human transcriptome

Emiliano Dalla, Roberto Verardo, Dejan Lazarević, Luigi Marchionni, James F. Reid, Nabil Bahar, Enio Klarić, Giacomo Marcuzzi, Riccardo Marzio, Anna Belgrano, Danilo Licastro, Claudio Schneider

Research output: Contribution to journalArticle

Abstract

LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

Original languageEnglish (US)
Pages (from-to)967-970
Number of pages4
JournalComptes Rendus - Biologies
Volume326
Issue number10-11
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Gene Library
Transcriptome
transcriptome
Complementary DNA
Genes
Cells
Tissue
Molecular Sequence Annotation
unigenes
cDNA libraries
Libraries
Clone Cells
cell lines
developmental stages
Databases
clones
Cell Line
genes
methodology
tissues

Keywords

  • Full-length cDNA
  • Functional analysis
  • Human transcriptome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Developmental Biology

Cite this

LNCIB human full-length cDNAs collection : Towards a better comprehension of the human transcriptome. / Dalla, Emiliano; Verardo, Roberto; Lazarević, Dejan; Marchionni, Luigi; Reid, James F.; Bahar, Nabil; Klarić, Enio; Marcuzzi, Giacomo; Marzio, Riccardo; Belgrano, Anna; Licastro, Danilo; Schneider, Claudio.

In: Comptes Rendus - Biologies, Vol. 326, No. 10-11, 10.2003, p. 967-970.

Research output: Contribution to journalArticle

Dalla, E, Verardo, R, Lazarević, D, Marchionni, L, Reid, JF, Bahar, N, Klarić, E, Marcuzzi, G, Marzio, R, Belgrano, A, Licastro, D & Schneider, C 2003, 'LNCIB human full-length cDNAs collection: Towards a better comprehension of the human transcriptome', Comptes Rendus - Biologies, vol. 326, no. 10-11, pp. 967-970. https://doi.org/10.1016/j.crvi.2003.09.029
Dalla, Emiliano ; Verardo, Roberto ; Lazarević, Dejan ; Marchionni, Luigi ; Reid, James F. ; Bahar, Nabil ; Klarić, Enio ; Marcuzzi, Giacomo ; Marzio, Riccardo ; Belgrano, Anna ; Licastro, Danilo ; Schneider, Claudio. / LNCIB human full-length cDNAs collection : Towards a better comprehension of the human transcriptome. In: Comptes Rendus - Biologies. 2003 ; Vol. 326, No. 10-11. pp. 967-970.
@article{79c360eedcfb4a4e92eb3c4cb5bb2b36,
title = "LNCIB human full-length cDNAs collection: Towards a better comprehension of the human transcriptome",
abstract = "LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.",
keywords = "Full-length cDNA, Functional analysis, Human transcriptome",
author = "Emiliano Dalla and Roberto Verardo and Dejan Lazarević and Luigi Marchionni and Reid, {James F.} and Nabil Bahar and Enio Klarić and Giacomo Marcuzzi and Riccardo Marzio and Anna Belgrano and Danilo Licastro and Claudio Schneider",
year = "2003",
month = "10",
doi = "10.1016/j.crvi.2003.09.029",
language = "English (US)",
volume = "326",
pages = "967--970",
journal = "Comptes Rendus - Biologies",
issn = "1631-0691",
publisher = "Elsevier Masson",
number = "10-11",

}

TY - JOUR

T1 - LNCIB human full-length cDNAs collection

T2 - Towards a better comprehension of the human transcriptome

AU - Dalla, Emiliano

AU - Verardo, Roberto

AU - Lazarević, Dejan

AU - Marchionni, Luigi

AU - Reid, James F.

AU - Bahar, Nabil

AU - Klarić, Enio

AU - Marcuzzi, Giacomo

AU - Marzio, Riccardo

AU - Belgrano, Anna

AU - Licastro, Danilo

AU - Schneider, Claudio

PY - 2003/10

Y1 - 2003/10

N2 - LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

AB - LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

KW - Full-length cDNA

KW - Functional analysis

KW - Human transcriptome

UR - http://www.scopus.com/inward/record.url?scp=9144273280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9144273280&partnerID=8YFLogxK

U2 - 10.1016/j.crvi.2003.09.029

DO - 10.1016/j.crvi.2003.09.029

M3 - Article

VL - 326

SP - 967

EP - 970

JO - Comptes Rendus - Biologies

JF - Comptes Rendus - Biologies

SN - 1631-0691

IS - 10-11

ER -