Liver-Targeted gene delivery through retrograde intrabiliary infusion

Xuan Jiang, Yong Ren, John Michael Williford, Zhiping Li, Guangzhao Mao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Retrograde intrabiliary infusion (RII) has recently been characterized as a safe and effective administration route for liver-Targeted gene delivery. Ef fi cient transgene expression in the liver has been achieved by infusing a variety of gene vectors including adenovirus, retrovirus, lipoplexes, polyplexes, and naked DNA through the common bile duct. Here, we describe the RII technique and key infusion parameters for delivering plasmid DNA and DNA nanoparticles to the rat liver. After RII of plasmid DNA, the level of transgene expression in rat liver is comparable to that achieved by hydrodynamic injection of plasmid DNA, which is considered to be "gold standard" for liver-Targeted gene delivery. RII has also been shown to signi fi cantly enhance the gene delivery ef fi ciency by polymer/DNA nanoparticles in comparison with intravenous and intraportal infusions. This method induces minimal level of cytotoxicity and damage to the liver and bile duct. Due to these advantages, RII has the potential to be used for delivering various gene vectors in clinical setting through the endoscopic retrograde cholangiopancreatography procedure.

Original languageEnglish (US)
Title of host publicationNanotechnology for Nucleic Acid Delivery
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages275-284
Number of pages10
ISBN (Print)9781627031394
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume948
ISSN (Print)1064-3745

Keywords

  • Liver-Targeted gene delivery
  • Plasmid DNA
  • Retrograde intrabiliary infusion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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