Liver from bone marrow in humans

Neil D. Theise, Manjunath Nimmakayalu, Rebekah Gardner, Peter B Illei, Glyn Morgan, Lewis Teperman, Octavian Henegariu, Diane S. Krause

Research output: Contribution to journalArticle

Abstract

It has been shown in animal models that hepatocytes and cholangiocytes can derive from bone marrow cells. We have investigated whether such a process occurs in humans. Archival autopsy and biopsy liver specimens were obtained from 2 female recipients of therapeutic bone marrow transplantations with male donors and from 4 male recipients of orthotopic liver transplantations from female donors. Immunohistochemical staining with monoclonal antibody CAM5.2, specific for cytokeratins 8, 18, and 19, gave typical strong staining of hepatocytes, cholangiocytes, and ductular reactions in all tissues, to the exclusion of all nonepithelial cells. Slides were systematically photographed and then restained by fluorescence in situ hybridization (FISH) for X and Y chromosomes. Using morphologic criteria, field-by-field comparison of the fluorescent images with the prior photomicrographs, and persistence of the diaminiobenzidene (DAB) stain through the FISH protease digestion, Y-positive hepatocytes and cholangiocytes could be identified in male control liver tissue and in all study specimens. Cell counts were adjusted based on the number of Y-positive cells in the male control liver to correct for partial sampling of nuclei in the 3-micron thin tissue sections. Adjusted Y-positive hepatocyte and cholangiocyte engraftment ranged from 4% to 43% and from 4% to 38%, respectively, in study specimens, with the peak values being found in a case of fibrosing cholestatic recurrent hepatitis C in one of the liver transplant recipients. We therefore show that in humans, hepatocytes and cholangiocytes can be derived from extrahepatic circulating stem cells, probably of bone marrow origin, and such 'transdifferentiation' can replenish large numbers of hepatic parenchymal cells.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalHepatology
Volume32
Issue number1
StatePublished - Jul 2000
Externally publishedYes

Fingerprint

Hepatocytes
Bone Marrow
Liver
Fluorescence In Situ Hybridization
Tissue Donors
Keratin-8
Staining and Labeling
Keratin-18
Keratin-19
Y Chromosome
X Chromosome
Hepatitis C
Bone Marrow Transplantation
Bone Marrow Cells
Liver Transplantation
Digestion
Autopsy
Peptide Hydrolases
Coloring Agents
Stem Cells

ASJC Scopus subject areas

  • Hepatology

Cite this

Theise, N. D., Nimmakayalu, M., Gardner, R., Illei, P. B., Morgan, G., Teperman, L., ... Krause, D. S. (2000). Liver from bone marrow in humans. Hepatology, 32(1), 11-16.

Liver from bone marrow in humans. / Theise, Neil D.; Nimmakayalu, Manjunath; Gardner, Rebekah; Illei, Peter B; Morgan, Glyn; Teperman, Lewis; Henegariu, Octavian; Krause, Diane S.

In: Hepatology, Vol. 32, No. 1, 07.2000, p. 11-16.

Research output: Contribution to journalArticle

Theise, ND, Nimmakayalu, M, Gardner, R, Illei, PB, Morgan, G, Teperman, L, Henegariu, O & Krause, DS 2000, 'Liver from bone marrow in humans', Hepatology, vol. 32, no. 1, pp. 11-16.
Theise ND, Nimmakayalu M, Gardner R, Illei PB, Morgan G, Teperman L et al. Liver from bone marrow in humans. Hepatology. 2000 Jul;32(1):11-16.
Theise, Neil D. ; Nimmakayalu, Manjunath ; Gardner, Rebekah ; Illei, Peter B ; Morgan, Glyn ; Teperman, Lewis ; Henegariu, Octavian ; Krause, Diane S. / Liver from bone marrow in humans. In: Hepatology. 2000 ; Vol. 32, No. 1. pp. 11-16.
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