Live imaging of the drosophila testis stem cell niche

Leah J. Greenspan, Erika Matunis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Live imaging of adult tissue stem cell niches provides key insights into the dynamic behavior of stem cells, their differentiating progeny, and their neighboring support cells, but few niches are amenable to this approach. Here we discuss a technique for long-term live imaging of the Drosophila testis stem cell niche. Culturing whole testes ex vivo for up to 12.5 h allows for tracking of cell-type specific behaviors under normal and various chemically or genetically modified conditions. Fixing and staining tissues after live imaging allows for the molecular confirmation of cell identity and behavior. Utilization of live imaging in intact niches will facilitate further understanding of the cellular and molecular mechanisms that regulate stem cell function in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages63-74
Number of pages12
Volume1463
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

Fingerprint

Stem Cell Niche
Drosophila
Testis
Stem Cells
Cell Tracking
Adult Stem Cells
Molecular Imaging
Staining and Labeling

Keywords

  • Confocal microscopy
  • Drosophila testis
  • Fluorescence
  • Niche
  • Stem cell
  • Time-lapse live imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Greenspan, L. J., & Matunis, E. (2017). Live imaging of the drosophila testis stem cell niche. In Methods in Molecular Biology (Vol. 1463, pp. 63-74). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_4

Live imaging of the drosophila testis stem cell niche. / Greenspan, Leah J.; Matunis, Erika.

Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. p. 63-74 (Methods in Molecular Biology; Vol. 1463).

Research output: Chapter in Book/Report/Conference proceedingChapter

Greenspan, LJ & Matunis, E 2017, Live imaging of the drosophila testis stem cell niche. in Methods in Molecular Biology. vol. 1463, Methods in Molecular Biology, vol. 1463, Humana Press Inc., pp. 63-74. https://doi.org/10.1007/978-1-4939-4017-2_4
Greenspan LJ, Matunis E. Live imaging of the drosophila testis stem cell niche. In Methods in Molecular Biology. Vol. 1463. Humana Press Inc. 2017. p. 63-74. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-4017-2_4
Greenspan, Leah J. ; Matunis, Erika. / Live imaging of the drosophila testis stem cell niche. Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. pp. 63-74 (Methods in Molecular Biology).
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