@inbook{2e00fef9bc574312b63700af4237eca3,
title = "Live imaging of the drosophila testis stem cell niche",
abstract = "Live imaging of adult tissue stem cell niches provides key insights into the dynamic behavior of stem cells, their differentiating progeny, and their neighboring support cells, but few niches are amenable to this approach. Here we discuss a technique for long-term live imaging of the Drosophila testis stem cell niche. Culturing whole testes ex vivo for up to 12.5 h allows for tracking of cell-type specific behaviors under normal and various chemically or genetically modified conditions. Fixing and staining tissues after live imaging allows for the molecular confirmation of cell identity and behavior. Utilization of live imaging in intact niches will facilitate further understanding of the cellular and molecular mechanisms that regulate stem cell function in vivo.",
keywords = "Confocal microscopy, Drosophila testis, Fluorescence, Niche, Stem cell, Time-lapse live imaging",
author = "Greenspan, {Leah J.} and Matunis, {Erika L.}",
note = "Funding Information: The authors thank Steve DiNardo and Kari Lenhart for sharing adaptations to the protocol, and Margaret de Cuevas and Miriam Akeju for comments on the manuscript. This work was supported by NIH Grants HD040307 and HD052937 (EM). The Zeiss LSM 780 confocal microscope used in this study was funded by NIH Grant S10 OD016374. LJG was supported by T32 GM007445. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-4017-2_4",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "63--74",
booktitle = "Methods in Molecular Biology",
}