Live imaging of border cell migration in Drosophila

Wei Dai, Denise J. Montell

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Border cells are a cluster of cells that migrate from the anterior tip of the Drosophila egg chamber to the border of the oocyte in stage 9. They serve as a useful model to study collective cell migration in a native tissue environment. Here we describe a protocol for preparing ex vivo egg chamber cultures from transgenic flies expressing fluorescent proteins in the border cells, and using confocal microscopy to take a multi-positional time-lapse movie. We include an image analysis method for tracking border cell cluster dynamics as well as tracking individual cell movements.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-168
Number of pages16
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1407
ISSN (Print)1064-3745

Keywords

  • 3D tracking
  • Border cell
  • Confocal
  • Drosophila
  • Egg chamber
  • Live imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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