@inbook{8b0eff03758b47fea5e9b72f8c0bd594,
title = "Live imaging of border cell migration in Drosophila",
abstract = "Border cells are a cluster of cells that migrate from the anterior tip of the Drosophila egg chamber to the border of the oocyte in stage 9. They serve as a useful model to study collective cell migration in a native tissue environment. Here we describe a protocol for preparing ex vivo egg chamber cultures from transgenic flies expressing fluorescent proteins in the border cells, and using confocal microscopy to take a multi-positional time-lapse movie. We include an image analysis method for tracking border cell cluster dynamics as well as tracking individual cell movements.",
keywords = "3D tracking, Border cell, Confocal, Drosophila, Egg chamber, Live imaging",
author = "Wei Dai and Montell, {Denise J.}",
note = "Funding Information: This work was supported by NIH grants R01-GM046425 and R01-GM073164 to DJM. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "2016",
doi = "10.1007/978-1-4939-3480-5_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "153--168",
booktitle = "Methods in Molecular Biology",
}