Live computerized videomicroscopy of cerebral microvessels in brain slices

Oren Sagher, Xi Qing Zhang, Wilson Szeto, Quoc Anh Thai, Yongcheng Jin, Neal F. Kassell, Kevin S. Lee

Research output: Contribution to journalArticle

Abstract

A model system for studying cerebral microvasculature is presented in which submerged in vitro brain slices are examined by computerized videomicroscopy. Brain slices are superfused continuously with artificial cerebrospinal fluid, while blood vessels are monitored using a transmission light microscope with water immersion objectives. The responses to well-characterized vasoactive compounds indicate that basic physiological characteristics are maintained in this preparation. This model system represents a simple and rapid technique for studying cerebrovascular responses under conditions in which vessels are surrounded by their normal cellular microenvironment. An additional advantage of this technique is the ability to perform simultaneous electrophysiological recordings in identified neurons. This will facilitate the study of interactions between neuronal and vascular elements and may help elucidate mechanisms underlying the local regulation of cerebral microvasculature.

Original languageEnglish (US)
Pages (from-to)676-682
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume13
Issue number4
DOIs
StatePublished - Jan 1 1993

Keywords

  • Brain slices
  • Microvasculature
  • Videomicroscopy

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Sagher, O., Zhang, X. Q., Szeto, W., Thai, Q. A., Jin, Y., Kassell, N. F., & Lee, K. S. (1993). Live computerized videomicroscopy of cerebral microvessels in brain slices. Journal of Cerebral Blood Flow and Metabolism, 13(4), 676-682. https://doi.org/10.1038/jcbfm.1993.86