Live-cell microrheology

Research output: Contribution to conferencePaper

Abstract

Cell migration proceeds through the highly coordinated translation of biochemical signals into specific biomechanical events. The biochemical and structural properties of the proteins involved in cell motility, as well as their subcellular localization, have been studied extensively. However, how these proteins work in concert to generate the mechanical properties required to produce global motility is not well understood. Using the method of particle-tracking microrheology applied to single living cells, we show that cytoskeleton reorganization produced by motility events results in regional stiffening of the cytoplasm of motile cells. We demonstrate that small GTPases Rho, Rac, and Cdc42 and their downstream effectors regulate cell mechanics and in turn control cell motility. We will also discuss recent advances in microrheology to compare the micromechanics of migrating fibroblasts in twodimensional vs. three-dimensional matrices and study the intracellular mechanical response of endothelial cells subjected to shear flows.

Original languageEnglish (US)
Number of pages1
StatePublished - Dec 1 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint Dive into the research topics of 'Live-cell microrheology'. Together they form a unique fingerprint.

  • Cite this

    Wirtz, D. (2005). Live-cell microrheology. Paper presented at 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States.