TY - JOUR
T1 - Listeria monocytogenes
T2 - A live vector able to deliver heterologous protein within the cytosol and to drive a CD8 dependent T cell response
AU - Goossens, Pierre L.
AU - Montixi, Christine
AU - Saron, Marie Françoise
AU - Rodriguez, Mauricio
AU - Zavala, Fidel
AU - Milon, Geneviève
N1 - Funding Information:
The enthusiasm and expertise of Pascale Cossart are greatly appreciated. We wish to thank Chantal Brulé-Courcy for her efficient and invaluable editing of the manuscript, L. Seigneur for technical assistance, M. Dehbi, E. Maranghi, A. Membrillera, K. Sebastien for their expertise with mice. This work was supported by Transdisease Vaccinology WHO/UNDP Programme for Vaccine Development and UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (PI. Pas-cale Cossart), as well as from Institut Pasteur (Regular funding and Comité Consultatif des Applications de la Recherche).
PY - 1995/6
Y1 - 1995/6
N2 - After an introduction on the entry and lifestyle ofListeria monocytogeneswithin mammalian eucaryotic cells, this chapter gives a brief overview of murine experimental listeriosis. Among the main characteristics of this murine model of infectious/pathogenic processes initiated by a facultative intracellular bacteria, we point out two main recent advances. One relates toListeria monocytogenes-induced production of cytokines as local, and transient signals able to direct the immune responses along a type 1 pathway of CD4/CD8 T cell differentiation. The other relates (a) to the recognition ofL. monocytogenes-reactive CD8+T lymphocytes as effectors able, once recruited within infected loci, to critically contribute to the complete clearance of the bacteria, and (b) to the recently recognized specificity of some of these CD8 lymphocytes in BALB/c mice. In this paper, we also briefly review (a) the readout assays presently used to monitor the outcome of the infectious/pathogenic processes and the related development and expression of immune responses induced by intravenous inoculation of wild-type virulent or attenuatedL. monocytogenes, (b) why all this information allows us to consider the use ofL. monocytogenesof attenuated virulence as relevant live recombinant vectors in order to deliver heterologous proteins to the class I processing and presentation pathway, and to induce CD8 T cells along the type 1 pathway.
AB - After an introduction on the entry and lifestyle ofListeria monocytogeneswithin mammalian eucaryotic cells, this chapter gives a brief overview of murine experimental listeriosis. Among the main characteristics of this murine model of infectious/pathogenic processes initiated by a facultative intracellular bacteria, we point out two main recent advances. One relates toListeria monocytogenes-induced production of cytokines as local, and transient signals able to direct the immune responses along a type 1 pathway of CD4/CD8 T cell differentiation. The other relates (a) to the recognition ofL. monocytogenes-reactive CD8+T lymphocytes as effectors able, once recruited within infected loci, to critically contribute to the complete clearance of the bacteria, and (b) to the recently recognized specificity of some of these CD8 lymphocytes in BALB/c mice. In this paper, we also briefly review (a) the readout assays presently used to monitor the outcome of the infectious/pathogenic processes and the related development and expression of immune responses induced by intravenous inoculation of wild-type virulent or attenuatedL. monocytogenes, (b) why all this information allows us to consider the use ofL. monocytogenesof attenuated virulence as relevant live recombinant vectors in order to deliver heterologous proteins to the class I processing and presentation pathway, and to induce CD8 T cells along the type 1 pathway.
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U2 - 10.1006/biol.1995.0024
DO - 10.1006/biol.1995.0024
M3 - Article
C2 - 7546656
AN - SCOPUS:0029006758
VL - 23
SP - 135
EP - 143
JO - Journal of Biological Standardization
JF - Journal of Biological Standardization
SN - 1045-1056
IS - 2
ER -