A reversed-phase ion-pairing liquid chromatography method with UV detection at 210 nm is described for the separation of lincomycin from 7-epilincomycin and lincomycin B. The method utilizes a base deactivated Supelcosil LC-ABZ, C-18, 5 μm, 250 × 4.6 mm i.d. column maintained at 45°C. The separation of lincomycin from 7-epilincomycin was only possible on a base deactivated column. This column was chosen after the octadecylsilyl columns examined showed no selectivity. Other base deactivated columns (Spherisorb S5-ODS-B, 5 μm, Hypersil BDS, C-18, 5 μm and Supelcosil LC-ABZ, C-18, 5 μm, all 250 × 4.6 mm i.d) examined also showed good selectivity. The mobile phase comprised 2.25 % v/v acetonitrile, 5 % v/v phosphate buffer (2.72 % m/v KH2PO4 adjusted to pH 5.0 with 3.48 % m/v K2HPO4), 0.0676 % v/v methane sulphonic acid and water to 100 %. The flow rate was 1.0 ml/min. The method showed good selectivity, linearity and repeatability.
|Original language||English (US)|
|Number of pages||1|
|Journal||Journal de Pharmacie de Belgique|
|State||Published - Dec 1 1998|
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