Liquid chromatography method for separation of lincomycin from its related substances

J. A. Orwa, F. Bosmans, S. Depuydt, E. Roets, J. Hoogmartens

Research output: Contribution to journalArticle

Abstract

A reversed-phase ion-pairing liquid chromatography method with UV detection at 210 nm is described for the separation of lincomycin from 7-epilincomycin and lincomycin B. The method utilizes a base deactivated Supelcosil LC-ABZ, C-18, 5 μm, 250 × 4.6 mm i.d. column maintained at 45°C. The separation of lincomycin from 7-epilincomycin was only possible on a base deactivated column. This column was chosen after the octadecylsilyl columns examined showed no selectivity. Other base deactivated columns (Spherisorb S5-ODS-B, 5 μm, Hypersil BDS, C-18, 5 μm and Supelcosil LC-ABZ, C-18, 5 μm, all 250 × 4.6 mm i.d) examined also showed good selectivity. The mobile phase comprised 2.25 % v/v acetonitrile, 5 % v/v phosphate buffer (2.72 % m/v KH2PO4 adjusted to pH 5.0 with 3.48 % m/v K2HPO4), 0.0676 % v/v methane sulphonic acid and water to 100 %. The flow rate was 1.0 ml/min. The method showed good selectivity, linearity and repeatability.

Original languageEnglish (US)
Number of pages1
JournalJournal de Pharmacie de Belgique
Volume53
Issue number3
StatePublished - Dec 1 1998

ASJC Scopus subject areas

  • Medicine(all)

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    Orwa, J. A., Bosmans, F., Depuydt, S., Roets, E., & Hoogmartens, J. (1998). Liquid chromatography method for separation of lincomycin from its related substances. Journal de Pharmacie de Belgique, 53(3).