Liquid chromatographic method for separation of lincomycin from its related substances

J. A. Orwa, F. Bosmans, S. Depuydt, E. Roets, J. Hoogmartens

Research output: Contribution to journalArticlepeer-review

Abstract

A reversed-phase ion-pair liquid chromatographic method with UV detection at 210 nm is described for the separation of lincomycin from 7-epilincomycin and lincomycin B. The method utilizes a base-deactivated Supelcosil LC-ABZ, C12-18, 5 μm, 250x4.6 mm I.D. column maintained at 45°C. The mobile phase consists of 2.25% (v/v) acetonitrile, 5% (v/v) phosphate buffer (2.72%, m/v KH2PO4 adjusted to pH 5.0 with 3.48%, m/v K2HPO4), 0.067% (v/v) methanesulfonic acid and water to 100%. The flow-rate is 1.0 ml/min. The separation of lincomycin from 7-epilincomycin is only possible on base-deactivated (BDS) columns. Other octadecylsilyl columns examined showed insufficient selectivity. The method was also tested on other BDS columns (Spherisorb S5-ODS-B, 5 μm, Hypersil BDS, C18, 5 μm and Supelcosil LC-ABZ, C18, 5 μm, all 250x4.6 mm I.D.) and showed good robustness. Robustness was further evaluated by performing a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity. It is also suitable for analysis of lincomycin formulations. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalJournal of Chromatography A
Volume829
Issue number1-2
DOIs
StatePublished - Dec 1 1998

Keywords

  • Antibiotics
  • Lincomycins
  • Pharmaceutical analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Liquid chromatographic method for separation of lincomycin from its related substances'. Together they form a unique fingerprint.

Cite this