Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain

Melanie Mueller; Frank T. Peters; George A. Ricaurte; Hans H. Maurer

doi:10.1016/j.jchromb.2008.09.009

Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain

Melanie Mueller, Frank T. Peters, George A. Ricaurte, Hans H. Maurer

School of Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

3,4-Methylenedioxymethamphetamine (MDMA) is a psychoactive drug with abuse liability and neurotoxic potential. Mechanisms by which MDMA produces behavioral and neurotoxic effects have yet to be elucidated. By measuring concentrations of MDMA and its metabolites in relevant brain sites, it may be possible to gain insight into mechanisms underlying MDMA actions. For this purpose, an LC-MS assay with electrospray ionization was developed after homogenization of rat brain and enzymatic conjugate cleavage. The method was successfully validated with respect to selectivity, linearity, accuracy, precision, recovery, and matrix effect and its use should help to delineate the neurotoxic mechanism of action of MDMA.

Original language	English (US)
Pages (from-to)	119-124
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	874
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2008.09.009
State	Published - Oct 15 2008

Keywords

Brain
LC-ESI-MS
MDMA
Metabolites
Neurotoxicity
Rat
Validation

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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Mueller, M., Peters, F. T., Ricaurte, G. A., & Maurer, H. H. (2008). Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 874(1-2), 119-124. https://doi.org/10.1016/j.jchromb.2008.09.009

Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain. / Mueller, Melanie; Peters, Frank T.; Ricaurte, George A. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 874, No. 1-2, 15.10.2008, p. 119-124.

Research output: Contribution to journal › Article › peer-review

Mueller, M, Peters, FT, Ricaurte, GA & Maurer, HH 2008, 'Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 874, no. 1-2, pp. 119-124. https://doi.org/10.1016/j.jchromb.2008.09.009

Mueller M, Peters FT, Ricaurte GA, Maurer HH. Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008 Oct 15;874(1-2):119-124. doi: 10.1016/j.jchromb.2008.09.009

Mueller, Melanie ; Peters, Frank T. ; Ricaurte, George A. et al. / Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008 ; Vol. 874, No. 1-2. pp. 119-124.

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abstract = "3,4-Methylenedioxymethamphetamine (MDMA) is a psychoactive drug with abuse liability and neurotoxic potential. Mechanisms by which MDMA produces behavioral and neurotoxic effects have yet to be elucidated. By measuring concentrations of MDMA and its metabolites in relevant brain sites, it may be possible to gain insight into mechanisms underlying MDMA actions. For this purpose, an LC-MS assay with electrospray ionization was developed after homogenization of rat brain and enzymatic conjugate cleavage. The method was successfully validated with respect to selectivity, linearity, accuracy, precision, recovery, and matrix effect and its use should help to delineate the neurotoxic mechanism of action of MDMA.",

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author = "Melanie Mueller and Peters, {Frank T.} and Ricaurte, {George A.} and Maurer, {Hans H.}",

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Liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in rat brain

Abstract

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