Lipoxygenase products in inflammatory synovial fluids and other exudates

Patrick B. Costello, Alan N. Baer, Floyd A. Green

Research output: Contribution to journalArticlepeer-review

Abstract

Forty six synovial fluid samples from 42 patients with inflammatory joint disease were analysed by reversed phase high performance liquid chromatography to determine 5-lipoxygenase products, specifically dihydroxyeicosatetraenoic acids (diHETEs). Twenty eight per cent of the fluids which were assayed had one or more products of 5-lipoxygenase activation. Seven fluids contained leukotriene B4 (0-1-28-1 ng/ml); three fluids had low concentrations of 20 carboxy/hydroxy-leukotriene B4 (0-01-0-05 ng/ml); three samples had leukotriene B4 isomers (1-5-2-4 ng/ml); and four fluids contained 5,15-diHETE (2.3-16-4 ng/ml). There was a poor correlation between synovial fluid white blood cell counts and evidence of 5-lipoxygenase activation. Several fluids contained unidentified compounds with spectra similar in shape to that of trienes, but the X.,,, values of these unidentified compounds were different from those of known leukotrienes. A septic peritoneal exudate and a septic pleural fluid had concentrations of leukotriene B4 and leukotriene B4 isomers and metabolites in a range similar to those found in synovial fluids.

Original languageEnglish (US)
Pages (from-to)1215-1218
Number of pages4
JournalAnnals of the rheumatic diseases
Volume51
Issue number11
DOIs
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

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