Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment

Sonja von Aulock, Siegfried Morath, Lars Hareng, Sylvia Knapp, Kok P M van Kessel, Jos A G van Strijp, Thomas Hartung

Research output: Contribution to journalArticle

Abstract

Lipoteichoic acid (LTA) is a major immunostimulatory principle of Gram-positive bacteria. Intranasal application of LTA from S. aureus to mice resulted in greatly increased neutrophil and macrophage counts in the bronchoalveolar lavage as well as increased levels of the chemokine KC. The potential of highly pure, bioactive LTA from S. aureus to induce neutrophil recruitment and activation was investigated further in the human system. Although neutrophils expressed the key known receptors, CD14, TLR2 and TLR6, LTA did not induce or prime neutrophils for oxidative burst, or release of chemokines, bactericidal permeability-increasing protein or myeloperoxidase. However, LTA induced a strong release of the chemoattractants LTB4, IL-8, C5a, MCP-1 and the colony-stimulating factor G-CSF in whole blood comparable to stimulation with the same concentration of LPS (S. abortus equi). Further, the cytokine and chemoattractant pattern induced by LTA correlated well with that induced by live S. aureus of the same strain. LTA does not appear to activate neutrophils directly, but is a strong stimulus for the recruitment of phagocytes to the site of infection.

Original languageEnglish (US)
Pages (from-to)413-422
Number of pages10
JournalImmunobiology
Volume208
Issue number4
DOIs
StatePublished - 2003
Externally publishedYes

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Neutrophil Infiltration
Staphylococcus aureus
Neutrophils
Chemotactic Factors
Chemokines
Toll-Like Receptor 6
Toll-Like Receptor 2
Neutrophil Activation
Macrophage Colony-Stimulating Factor
Leukotriene B4
Respiratory Burst
Gram-Positive Bacteria
Bronchoalveolar Lavage
Granulocyte Colony-Stimulating Factor
Phagocytes
lipoteichoic acid
Interleukin-8
Peroxidase
Macrophages
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

von Aulock, S., Morath, S., Hareng, L., Knapp, S., van Kessel, K. P. M., van Strijp, J. A. G., & Hartung, T. (2003). Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment. Immunobiology, 208(4), 413-422. https://doi.org/10.1078/0171-2985-00285

Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment. / von Aulock, Sonja; Morath, Siegfried; Hareng, Lars; Knapp, Sylvia; van Kessel, Kok P M; van Strijp, Jos A G; Hartung, Thomas.

In: Immunobiology, Vol. 208, No. 4, 2003, p. 413-422.

Research output: Contribution to journalArticle

von Aulock, S, Morath, S, Hareng, L, Knapp, S, van Kessel, KPM, van Strijp, JAG & Hartung, T 2003, 'Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment', Immunobiology, vol. 208, no. 4, pp. 413-422. https://doi.org/10.1078/0171-2985-00285
von Aulock S, Morath S, Hareng L, Knapp S, van Kessel KPM, van Strijp JAG et al. Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment. Immunobiology. 2003;208(4):413-422. https://doi.org/10.1078/0171-2985-00285
von Aulock, Sonja ; Morath, Siegfried ; Hareng, Lars ; Knapp, Sylvia ; van Kessel, Kok P M ; van Strijp, Jos A G ; Hartung, Thomas. / Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment. In: Immunobiology. 2003 ; Vol. 208, No. 4. pp. 413-422.
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