TY - JOUR
T1 - Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment
AU - von Aulock, Sonja
AU - Morath, Siegfried
AU - Hareng, Lars
AU - Knapp, Sylvia
AU - van Kessel, Kok P.M.
AU - van Strijp, Jos A.G.
AU - Hartung, Thomas
N1 - Funding Information:
Acknowledgements. The authors would like to thank Erik Heezius, Susanne Deininger, Stephanie Traub and Rebek-ka Munke for their prompt help and Alexandra Pfeiffer for helpful advice on TLR staining. This work was supported by the German Research Council (DFG) Schwerpunkt program ™Innate Immunity∫ HA 2567/9-1 and a grant awarded to S. Knapp by the Austrian Fonds zur Foerde-rung des wissenschaftlichen Forschung in Oesterreich.
PY - 2003
Y1 - 2003
N2 - Lipoteichoic acid (LTA) is a major immunostimulatory principle of Gram-positive bacteria. Intranasal application of LTA from S. aureus to mice resulted in greatly increased neutrophil and macrophage counts in the bronchoalveolar lavage as well as increased levels of the chemokine KC. The potential of highly pure, bioactive LTA from S. aureus to induce neutrophil recruitment and activation was investigated further in the human system. Although neutrophils expressed the key known receptors, CD14, TLR2 and TLR6, LTA did not induce or prime neutrophils for oxidative burst, or release of chemokines, bactericidal permeability-increasing protein or myeloperoxidase. However, LTA induced a strong release of the chemoattractants LTB4, IL-8, C5a, MCP-1 and the colony-stimulating factor G-CSF in whole blood comparable to stimulation with the same concentration of LPS (S. abortus equi). Further, the cytokine and chemoattractant pattern induced by LTA correlated well with that induced by live S. aureus of the same strain. LTA does not appear to activate neutrophils directly, but is a strong stimulus for the recruitment of phagocytes to the site of infection.
AB - Lipoteichoic acid (LTA) is a major immunostimulatory principle of Gram-positive bacteria. Intranasal application of LTA from S. aureus to mice resulted in greatly increased neutrophil and macrophage counts in the bronchoalveolar lavage as well as increased levels of the chemokine KC. The potential of highly pure, bioactive LTA from S. aureus to induce neutrophil recruitment and activation was investigated further in the human system. Although neutrophils expressed the key known receptors, CD14, TLR2 and TLR6, LTA did not induce or prime neutrophils for oxidative burst, or release of chemokines, bactericidal permeability-increasing protein or myeloperoxidase. However, LTA induced a strong release of the chemoattractants LTB4, IL-8, C5a, MCP-1 and the colony-stimulating factor G-CSF in whole blood comparable to stimulation with the same concentration of LPS (S. abortus equi). Further, the cytokine and chemoattractant pattern induced by LTA correlated well with that induced by live S. aureus of the same strain. LTA does not appear to activate neutrophils directly, but is a strong stimulus for the recruitment of phagocytes to the site of infection.
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U2 - 10.1078/0171-2985-00285
DO - 10.1078/0171-2985-00285
M3 - Article
C2 - 14748514
AN - SCOPUS:0347126573
SN - 0171-2985
VL - 208
SP - 413
EP - 422
JO - Immunobiology
JF - Immunobiology
IS - 4
ER -