Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat

Paul Y. Liu, Wenjing Tong, Kan Liu, Sang Hoon Han, Xiao Tian Wang, Evangelos Badiavas, Kimberly Rieger-Christ, Ian Summerhayes

Research output: Contribution to journalArticle

Abstract

Tissue engineering is an application for gene therapy that is in its infancy. We show that simple liposomal-mediated gene transfer could result in a potentially useful biological effect in the field of wound healing. cDNA encoding the 165 amino acid form of vascular endothelial growth factor complexed to commercially available liposomes was injected into rat skin 1 week before raising a random pattern 3 × 10 cm flap. The flap survival was enhanced by 14 percent, and was accomplished without accessing the arterial inflow of the territory. These results were statistically significant (p < 0.002) and reproducible. No adverse effects were seen. Histological analysis of the angiogenesis localized much of the new vessel formation to the area around the hair follicles. Polymerase chain reaction amplification of extracted flap tissue confirmed the presence of the transgene.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalWound Repair and Regeneration
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2004

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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