Liposome-mediated gene transfer into human basal cell carcinoma

M. O. Hottiger, T. N. Dam, B. J. Nickoloff, T. M. Johnson, G. J. Nabel

Research output: Contribution to journalArticlepeer-review

Abstract

Direct intralesional injection of DNA encoding interferon-a2 (IFN-a2) was used in an effort to sustain local protein delivery for the treatment of human basal cell carcinoma (BCC). A novel model to study this malignancy was established by transplantation of human BCC tissue on to immunodeficient mice with a relatively high rate of engraftment and stable phenotype for superficial BCC (20 of 25; 80). Gene transfer was significantly increased by using DNA liposome complexes (lipoplexes). Recombinant gene expression was detected predominantly in the epidermis and, to a lesser extent, in the dermis. Gene transfer of IFN-a2 using this method resulted in sustained production of IFN-a2 protein and increased expression of a known IFN-inducible gene, the class II major histocompatibility (MHC) antigen, and induced BCC regression, presumably through a non-immune mechanism. Intralesional injection of DNA lipoplexes encoding IFN-a protein may therefore be applicable to the treatment of cutaneous BCC.

Original languageEnglish (US)
Pages (from-to)1929-1935
Number of pages7
JournalGene Therapy
Volume6
Issue number12
DOIs
StatePublished - Dec 1999
Externally publishedYes

Keywords

  • Antigen-presenting cells
  • Basal cell carcinoma
  • Cationic liposomes
  • Gene therapy
  • Interferon
  • Lipoplexes

ASJC Scopus subject areas

  • Genetics

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