Liposome-based mucus-penetrating particles (MPP) for mucosal theranostics: Demonstration of diamagnetic chemical exchange saturation transfer (diaCEST) magnetic resonance imaging (MRI)

Tao Yu, Kannie W.Y. Chan, Abraham Anonuevo, Xiaolei Song, Benjamin S. Schuster, Sumon Chattopadhyay, Qingguo Xu, Nikita Oskolkov, Himatkumar Patel, Laura M. Ensign, Peter C.M. van Zjil, Michael T. McMahon, Justin Hanes

Research output: Contribution to journalArticle

Abstract

Mucus barriers lining mucosal epithelia reduce the effectiveness of nanocarrier-based mucosal drug delivery and imaging ("theranostics"). Here, we describe liposome-based mucus-penetrating particles (MPP) capable of loading hydrophilic agents, e.g., the diaCEST MRI contrast agent barbituric acid (BA). We observed that polyethylene glycol (PEG)-coated liposomes containing ≥. 7. mol% PEG diffused only ~. 10-fold slower in human cervicovaginal mucus (CVM) compared to their theoretical speeds in water. 7. mol%-PEG liposomes contained sufficient BA loading for diaCEST contrast, and provided improved vaginal distribution compared to 0 and 3. mol%-PEG liposomes. However, increasing PEG content to ~. 12. mol% compromised BA loading and vaginal distribution, suggesting that PEG content must be optimized to maintain drug loading and stability. Non-invasive diaCEST MRI illustrated uniform vaginal coverage and longer retention of BA-loaded 7. mol%-PEG liposomes compared to unencapsulated BA. Liposomal MPP with optimized PEG content hold promise for drug delivery and imaging at mucosal surfaces.

Original languageEnglish (US)
Pages (from-to)401-405
Number of pages5
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2015

Keywords

  • Barbituric acid
  • CEST
  • Drug and gene delivery
  • Lipid

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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