Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-κB activation, tumor necrosis factor-α production, and subsequent ischemia-induced apoptosis

Kirstan K. Meldrum, Arthur Burnett, Xianzhong Meng, Rosalia Misseri, Matthew B K Shaw, John Phillip Gearhart, Daniel R. Meldrum

Research output: Contribution to journalArticle

Abstract

Heat shock protein 72 (HSP72) is a stress-inducible protein capable of protecting a variety of cells from toxins, thermal stress, and ischemic injury. The cytoprotective role and mechanism of action of HSP72 in renal cell ischemic injury remain unclear. To study this, HSP72 was introduced (liposomal transfer) or induced (thermal stress, 43°C×1 hour) in renal tubular cells (LLC-PK1) with Western blot confirmation. Cells were subjected to simulated ischemia 24 hours after liposomal HSP72 transfer or thermal stress, and the effect of HSP72 on nuclear factor-κB (NF-κB) activation (electrophoretic mobility shift assay and immunohistochemistry), IκBα production (Western blot), postischemic tumor necrosis factor-α (TNF-α) production (RT-PCR), and apoptosis (TUNEL assay) were determined. In separate experiments, the role of TNF-α in apoptosis was determined (anti-TNF-α neutralizing antibody). Results demonstrated that both liposomal transfer of HSP72 and thermal induction of HSP72 prevented NF-κB activation and translocation, TNF-α gene transcription, and subsequent ischemia-induced renal tubular cell apoptosis. Furthermore, TNF-α neutralization also inhibited ischemia-induced renal tubular cell apoptosis. These results indicate that liposomal delivery of HSP72 inhibits ischemia-induced renal tubular cell apoptosis by preventing NF-κB activation and subsequent TNF-α production. Further elucidation of the mechanisms of HSP-induced cytoprotection may result in therapeutic strategies that limit or prevent ischemia-induced renal damage.

Original languageEnglish (US)
Pages (from-to)293-299
Number of pages7
JournalCirculation Research
Volume92
Issue number3
DOIs
StatePublished - Feb 21 2003

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HSP72 Heat-Shock Proteins
Ischemia
Tumor Necrosis Factor-alpha
Apoptosis
Kidney
Hot Temperature
Western Blotting
LLC-PK1 Cells
Cytoprotection
In Situ Nick-End Labeling
Wounds and Injuries
Electrophoretic Mobility Shift Assay
Heat-Shock Proteins
Neutralizing Antibodies
Immunohistochemistry
Polymerase Chain Reaction

Keywords

  • Inflammation
  • Kidney
  • Reperfusion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-κB activation, tumor necrosis factor-α production, and subsequent ischemia-induced apoptosis. / Meldrum, Kirstan K.; Burnett, Arthur; Meng, Xianzhong; Misseri, Rosalia; Shaw, Matthew B K; Gearhart, John Phillip; Meldrum, Daniel R.

In: Circulation Research, Vol. 92, No. 3, 21.02.2003, p. 293-299.

Research output: Contribution to journalArticle

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