Lipoprotein sub-fractions by ion-mobility analysis and its association with subclinical coronary atherosclerosis in high-risk individuals

Ehimen C. Aneni, Chukwuemeka U. Osondu, Javier De La Cruz, Seth Martin, Michael Blaha, Adnan Younus, Theodore Feldman, Arthur S. Agatston, Emir Veledar, Khurram Nasir

Research output: Contribution to journalArticle

Abstract

Aims: There is limited knowledge about the association of lipoprotein particles and markers of coronary atherosclerosis such as coronary artery calcification (CAC) in relatively young high-risk persons. This study examines the association of lipoprotein subfractions and CAC in high cardiometabolic risk individuals. Methods: The study presents analysis from baseline data of a randomized trial targeted at high-risk workers. Employees of Baptist Health South Florida with metabolic syndrome or diabetes were recruited. At baseline, all 182 participants had lipoprotein subfraction analysis using the ion mobility technique and participants above 35 years (N =170) had CAC test done. Principal components (PC) were computed for the combination of lipoprotein subclasses. Multiple bootstrapped regression analyses (BSA) were conducted to assess the relationship between lipoprotein subfractions and CAC. Results: The study population (N=170) was largely female (84%) with a mean age of 58 years. Three PCs accounted for 88% variation in the sample. PC2, with main contributions from VLDL particles in the positive direction and large LDL particles in the negative direction was associated with a 22% increase in CAC odds (P value <0.05 in 100% of BSA). PC3, with main contributions from HDL lipoprotein particles in the positive direction and small/medium LDL and large IDL particles in the negative direction, was associated with a 9% reduction in CAC odds (P<0.05 in 88% of BSA). PC1, which had approximately even contributions from HDL, LDL, IDL and VLDL lipoprotein subfractions in the positive direction, was not associated with CAC. Conclusion: In a relatively young but high-risk population, a lipoprotein profile predominated by triglyceride-rich lipoproteins was associated with increased risk of CAC, while one predominated by HDL lipoproteins offered modest protection. Lipoprotein sub-fraction analysis may help to further discriminate patients who require more intensive cardiovascular work-up and treatment.

Original languageEnglish (US)
Pages (from-to)50-63
Number of pages14
JournalJournal of Atherosclerosis and Thrombosis
Volume26
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Lipoproteins
Coronary Artery Disease
Coronary Vessels
Association reactions
Ions
HDL Lipoproteins
Regression Analysis
IDL Lipoproteins
VLDL Lipoproteins
Occupational Health
Medical problems
LDL Lipoproteins
Population
Triglycerides
Direction compound
Health
Personnel

Keywords

  • Coronary Atherosclerosis
  • CVD risk stratification
  • Ion-Mobility
  • Lipoprotein Subfractions
  • Principal Component Analysis

ASJC Scopus subject areas

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Biochemistry, medical

Cite this

Lipoprotein sub-fractions by ion-mobility analysis and its association with subclinical coronary atherosclerosis in high-risk individuals. / Aneni, Ehimen C.; Osondu, Chukwuemeka U.; De La Cruz, Javier; Martin, Seth; Blaha, Michael; Younus, Adnan; Feldman, Theodore; Agatston, Arthur S.; Veledar, Emir; Nasir, Khurram.

In: Journal of Atherosclerosis and Thrombosis, Vol. 26, No. 1, 01.01.2019, p. 50-63.

Research output: Contribution to journalArticle

Aneni, Ehimen C. ; Osondu, Chukwuemeka U. ; De La Cruz, Javier ; Martin, Seth ; Blaha, Michael ; Younus, Adnan ; Feldman, Theodore ; Agatston, Arthur S. ; Veledar, Emir ; Nasir, Khurram. / Lipoprotein sub-fractions by ion-mobility analysis and its association with subclinical coronary atherosclerosis in high-risk individuals. In: Journal of Atherosclerosis and Thrombosis. 2019 ; Vol. 26, No. 1. pp. 50-63.
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abstract = "Aims: There is limited knowledge about the association of lipoprotein particles and markers of coronary atherosclerosis such as coronary artery calcification (CAC) in relatively young high-risk persons. This study examines the association of lipoprotein subfractions and CAC in high cardiometabolic risk individuals. Methods: The study presents analysis from baseline data of a randomized trial targeted at high-risk workers. Employees of Baptist Health South Florida with metabolic syndrome or diabetes were recruited. At baseline, all 182 participants had lipoprotein subfraction analysis using the ion mobility technique and participants above 35 years (N =170) had CAC test done. Principal components (PC) were computed for the combination of lipoprotein subclasses. Multiple bootstrapped regression analyses (BSA) were conducted to assess the relationship between lipoprotein subfractions and CAC. Results: The study population (N=170) was largely female (84{\%}) with a mean age of 58 years. Three PCs accounted for 88{\%} variation in the sample. PC2, with main contributions from VLDL particles in the positive direction and large LDL particles in the negative direction was associated with a 22{\%} increase in CAC odds (P value <0.05 in 100{\%} of BSA). PC3, with main contributions from HDL lipoprotein particles in the positive direction and small/medium LDL and large IDL particles in the negative direction, was associated with a 9{\%} reduction in CAC odds (P<0.05 in 88{\%} of BSA). PC1, which had approximately even contributions from HDL, LDL, IDL and VLDL lipoprotein subfractions in the positive direction, was not associated with CAC. Conclusion: In a relatively young but high-risk population, a lipoprotein profile predominated by triglyceride-rich lipoproteins was associated with increased risk of CAC, while one predominated by HDL lipoproteins offered modest protection. Lipoprotein sub-fraction analysis may help to further discriminate patients who require more intensive cardiovascular work-up and treatment.",
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AU - Aneni, Ehimen C.

AU - Osondu, Chukwuemeka U.

AU - De La Cruz, Javier

AU - Martin, Seth

AU - Blaha, Michael

AU - Younus, Adnan

AU - Feldman, Theodore

AU - Agatston, Arthur S.

AU - Veledar, Emir

AU - Nasir, Khurram

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AB - Aims: There is limited knowledge about the association of lipoprotein particles and markers of coronary atherosclerosis such as coronary artery calcification (CAC) in relatively young high-risk persons. This study examines the association of lipoprotein subfractions and CAC in high cardiometabolic risk individuals. Methods: The study presents analysis from baseline data of a randomized trial targeted at high-risk workers. Employees of Baptist Health South Florida with metabolic syndrome or diabetes were recruited. At baseline, all 182 participants had lipoprotein subfraction analysis using the ion mobility technique and participants above 35 years (N =170) had CAC test done. Principal components (PC) were computed for the combination of lipoprotein subclasses. Multiple bootstrapped regression analyses (BSA) were conducted to assess the relationship between lipoprotein subfractions and CAC. Results: The study population (N=170) was largely female (84%) with a mean age of 58 years. Three PCs accounted for 88% variation in the sample. PC2, with main contributions from VLDL particles in the positive direction and large LDL particles in the negative direction was associated with a 22% increase in CAC odds (P value <0.05 in 100% of BSA). PC3, with main contributions from HDL lipoprotein particles in the positive direction and small/medium LDL and large IDL particles in the negative direction, was associated with a 9% reduction in CAC odds (P<0.05 in 88% of BSA). PC1, which had approximately even contributions from HDL, LDL, IDL and VLDL lipoprotein subfractions in the positive direction, was not associated with CAC. Conclusion: In a relatively young but high-risk population, a lipoprotein profile predominated by triglyceride-rich lipoproteins was associated with increased risk of CAC, while one predominated by HDL lipoproteins offered modest protection. Lipoprotein sub-fraction analysis may help to further discriminate patients who require more intensive cardiovascular work-up and treatment.

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