Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts

Alexandra Pfeiffer; Alfred Bttcher; Evelyn Ors; Michael Kapinsky; Peter Nagy; Andrea Bodnr; Ingo Spreitzer; Gerhard Liebisch; Wolfgang Drobnik; Klaus Gempel; Markus Horn; Stefan Holmer; Thomas Hartung; Gabriele Multhoff; Gerhard Schtz; Hansgeorg Schindler; Artur J. Ulmer; Holger Heine; Felix Stelter; Christine Schtt; Gregor Rothe; János Szöllősi; Sndor Damjanovich; Gerd Schmitz

doi:10.1002/1521-4141(200111)31:11<3153::AID-IMMU3153>3.0.CO;2-0

Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts

Alexandra Pfeiffer, Alfred Bttcher, Evelyn Ors, Michael Kapinsky, Peter Nagy, Andrea Bodnr, Ingo Spreitzer, Gerhard Liebisch, Wolfgang Drobnik, Klaus Gempel, Markus Horn, Stefan Holmer, Thomas Hartung, Gabriele Multhoff, Gerhard Schtz, Hansgeorg Schindler, Artur J. Ulmer, Holger Heine, Felix Stelter, Christine SchttGregor Rothe, János Szöllősi, Sndor Damjanovich, Gerd Schmitz

Research output: Contribution to journal › Article › peer-review

264 Scopus citations

Abstract

The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co-receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcγ-receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcγ-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14.

Original language	English (US)
Pages (from-to)	3153-3164
Number of pages	12
Journal	European Journal of Immunology
Volume	31
Issue number	11
DOIs	https://doi.org/10.1002/1521-4141(200111)31:11<3153::AID-IMMU3153>3.0.CO;2-0
State	Published - 2001
Externally published	Yes

Keywords

CD14
Ceramide
Lipopolysaccharide
Rafts
Receptor complexes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1002/1521-4141(200111)31:11<3153::AID-IMMU3153>3.0.CO;2-0

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Pfeiffer, A., Bttcher, A., Ors, E., Kapinsky, M., Nagy, P., Bodnr, A., Spreitzer, I., Liebisch, G., Drobnik, W., Gempel, K., Horn, M., Holmer, S., Hartung, T., Multhoff, G., Schtz, G., Schindler, H., Ulmer, A. J., Heine, H., Stelter, F., ... Schmitz, G. (2001). Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts. European Journal of Immunology, 31(11), 3153-3164. https://doi.org/10.1002/1521-4141(200111)31:11<3153::AID-IMMU3153>3.0.CO;2-0

Pfeiffer, A, Bttcher, A, Ors, E, Kapinsky, M, Nagy, P, Bodnr, A, Spreitzer, I, Liebisch, G, Drobnik, W, Gempel, K, Horn, M, Holmer, S, Hartung, T, Multhoff, G, Schtz, G, Schindler, H, Ulmer, AJ, Heine, H, Stelter, F, Schtt, C, Rothe, G, Szöllősi, J, Damjanovich, S & Schmitz, G 2001, 'Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts', European Journal of Immunology, vol. 31, no. 11, pp. 3153-3164. https://doi.org/10.1002/1521-4141(200111)31:11<3153::AID-IMMU3153>3.0.CO;2-0

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abstract = "The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co-receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcγ-receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcγ-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14.",

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author = "Alexandra Pfeiffer and Alfred Bttcher and Evelyn Ors and Michael Kapinsky and Peter Nagy and Andrea Bodnr and Ingo Spreitzer and Gerhard Liebisch and Wolfgang Drobnik and Klaus Gempel and Markus Horn and Stefan Holmer and Thomas Hartung and Gabriele Multhoff and Gerhard Schtz and Hansgeorg Schindler and Ulmer, {Artur J.} and Holger Heine and Felix Stelter and Christine Schtt and Gregor Rothe and J{\'a}nos Sz{\"o}ll{\H o}si and Sndor Damjanovich and Gerd Schmitz",

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T1 - Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts

AU - Pfeiffer, Alexandra

AU - Bttcher, Alfred

AU - Ors, Evelyn

AU - Kapinsky, Michael

AU - Nagy, Peter

AU - Bodnr, Andrea

AU - Spreitzer, Ingo

AU - Liebisch, Gerhard

AU - Drobnik, Wolfgang

AU - Gempel, Klaus

AU - Horn, Markus

AU - Holmer, Stefan

AU - Hartung, Thomas

AU - Multhoff, Gabriele

AU - Schtz, Gerhard

AU - Schindler, Hansgeorg

AU - Ulmer, Artur J.

AU - Heine, Holger

AU - Stelter, Felix

AU - Schtt, Christine

AU - Rothe, Gregor

AU - Szöllősi, János

AU - Damjanovich, Sndor

AU - Schmitz, Gerd

PY - 2001

Y1 - 2001

N2 - The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co-receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcγ-receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcγ-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14.

AB - The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co-receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcγ-receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcγ-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14.

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KW - Lipopolysaccharide

KW - Rafts

KW - Receptor complexes

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Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts

Abstract

Keywords

ASJC Scopus subject areas

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