Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts

Alexandra Pfeiffer, Alfred Bttcher, Evelyn Ors, Michael Kapinsky, Peter Nagy, Andrea Bodnr, Ingo Spreitzer, Gerhard Liebisch, Wolfgang Drobnik, Klaus Gempel, Markus Horn, Stefan Holmer, Thomas Hartung, Gabriele Multhoff, Gerhard Schtz, Hansgeorg Schindler, Artur J. Ulmer, Holger Heine, Felix Stelter, Christine SchttGregor Rothe, Jnos Szlli, Sndor Damjanovich, Gerd Schmitz

Research output: Contribution to journalArticle

Abstract

The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co-receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcγ-receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcγ-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14.

Original languageEnglish (US)
Pages (from-to)3153-3164
Number of pages12
JournalEuropean Journal of Immunology
Volume31
Issue number11
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Keywords

  • CD14
  • Ceramide
  • Lipopolysaccharide
  • Rafts
  • Receptor complexes

ASJC Scopus subject areas

  • Immunology

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