Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes

Marilia Kimie Shimabukuro, Larissa Gutman Paranhos Langhi, Ingrid Cordeiro, José M. Brito, Claudia Maria De Castro Batista, Mark P. Mattson, Valeria De Mello Coelho

Research output: Contribution to journalArticle

Abstract

We characterized cerebral Oil Red O-positive lipid-laden cells (LLC) of aging mice evaluating their distribution, morphology, density, functional activities and inflammatory phenotype. We identified LLC in meningeal, cortical and neurogenic brain regions. The density of cerebral LLC increased with age. LLC presenting small lipid droplets were visualized adjacent to blood vessels or deeper in the brain cortical and striatal parenchyma of aging mice. LLC with larger droplets were asymmetrically distributed in the cerebral ventricle walls, mainly located in the lateral wall. We also found that LLC in the subventricular region co-expressed beclin-1 or LC3, markers for autophagosome or autophagolysosome formation, and perilipin (PLIN), a lipid droplet-associated protein, suggesting lipophagic activity. Some cerebral LLC exhibited β galactosidase activity indicating a senescence phenotype. Moreover, we detected production of the pro-inflammatory cytokine TNF-α in cortical PLIN+ LLC. Some cortical NeuN+ neurons, GFAP+ glia limitans astrocytes, Iba-1+ microglia and S100β+ ependymal cells expressed PLIN in the aging brain. Our findings suggest that cerebral LLC exhibit distinct cellular phenotypes and may participate in the age-associated neuroinflammatory processes.

Original languageEnglish (US)
Article number23795
JournalScientific Reports
Volume6
DOIs
StatePublished - Mar 31 2016
Externally publishedYes

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Phenotype
Lipids
Brain
Galactosidases
Corpus Striatum
Cerebral Ventricles
Cell Aging
Microglia
Neuroglia
Astrocytes
Blood Vessels
Cytokines
Neurons
Perilipin-1

ASJC Scopus subject areas

  • General

Cite this

Shimabukuro, M. K., Langhi, L. G. P., Cordeiro, I., Brito, J. M., Batista, C. M. D. C., Mattson, M. P., & De Mello Coelho, V. (2016). Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes. Scientific Reports, 6, [23795]. https://doi.org/10.1038/srep23795

Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes. / Shimabukuro, Marilia Kimie; Langhi, Larissa Gutman Paranhos; Cordeiro, Ingrid; Brito, José M.; Batista, Claudia Maria De Castro; Mattson, Mark P.; De Mello Coelho, Valeria.

In: Scientific Reports, Vol. 6, 23795, 31.03.2016.

Research output: Contribution to journalArticle

Shimabukuro, MK, Langhi, LGP, Cordeiro, I, Brito, JM, Batista, CMDC, Mattson, MP & De Mello Coelho, V 2016, 'Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes', Scientific Reports, vol. 6, 23795. https://doi.org/10.1038/srep23795
Shimabukuro, Marilia Kimie ; Langhi, Larissa Gutman Paranhos ; Cordeiro, Ingrid ; Brito, José M. ; Batista, Claudia Maria De Castro ; Mattson, Mark P. ; De Mello Coelho, Valeria. / Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes. In: Scientific Reports. 2016 ; Vol. 6.
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