Linoleic acid activates nuclear transcription factor-κB (NF-κB) and induces NF-κB-dependent transcription in cultured endothelial cells

Bernhard Hennig, Michal Toborek, Swati Joshi-Barve, Steven W. Barger, Shirish Barve, Mark P. Mattson, Craig J. McClain

Research output: Contribution to journalArticle

Abstract

High dietary intakes of unsaturated fats may be atherogenic by disrupting normal functions of the vascular endothelium, due in part to the ability of linoleic acid (18:2n-6) to contribute to an increase in cellular oxidative stress and related injurious events. Exposing endothelial cells to 90 μmol linoleic acid/L for 6 h resulted in a significant increase in lipid hydroperoxides that coincided with an increase in intracellular calcium concentrations. Treatment with this fatty acid caused an initial decrease in glutathione concentrations, which was followed by an increase at later time points. Most importantly, a significant activation of the oxidative stress- sensitive nuclear transcription factor-κB (NF-κB) was achieved after a 6-h exposure to 18:2n-6, which is the time point at which maximal depletion of cellular glutathione was observed. The fatty acid-mediated NF-κB activation was accompanied by induction of NF-κB-dependent transcription, as measured by chloramphenicol acetyltransferase (CAT) assay of an NF-κB-responsive promoter construct. Pretreatment of endothelial cells with vitamin E and N- acetyl cysteine inhibited the fatty acid-induced activation of NF-κB and formation of lipid hydroperoxides. These data suggest that oxidative stress- induced cellular changes are critical early events in fatty acid-mediated endothelial cell dysfunction.

Original languageEnglish (US)
Pages (from-to)322-328
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume63
Issue number3
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Linoleic Acid
cultured cells
endothelial cells
linoleic acid
Cultured Cells
Transcription Factors
Endothelial Cells
transcription factors
transcription (genetics)
Fatty Acids
fatty acids
Oxidative Stress
oxidative stress
Lipid Peroxides
hydroperoxides
Glutathione
glutathione
chloramphenicol acetyltransferase
Unsaturated Dietary Fats
acetylcysteine

Keywords

  • endothelial dysfunction
  • Fatty acid
  • nuclear transcription factor- κB
  • transcription

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Hennig, B., Toborek, M., Joshi-Barve, S., Barger, S. W., Barve, S., Mattson, M. P., & McClain, C. J. (1996). Linoleic acid activates nuclear transcription factor-κB (NF-κB) and induces NF-κB-dependent transcription in cultured endothelial cells. American Journal of Clinical Nutrition, 63(3), 322-328.

Linoleic acid activates nuclear transcription factor-κB (NF-κB) and induces NF-κB-dependent transcription in cultured endothelial cells. / Hennig, Bernhard; Toborek, Michal; Joshi-Barve, Swati; Barger, Steven W.; Barve, Shirish; Mattson, Mark P.; McClain, Craig J.

In: American Journal of Clinical Nutrition, Vol. 63, No. 3, 03.1996, p. 322-328.

Research output: Contribution to journalArticle

Hennig, B, Toborek, M, Joshi-Barve, S, Barger, SW, Barve, S, Mattson, MP & McClain, CJ 1996, 'Linoleic acid activates nuclear transcription factor-κB (NF-κB) and induces NF-κB-dependent transcription in cultured endothelial cells', American Journal of Clinical Nutrition, vol. 63, no. 3, pp. 322-328.
Hennig, Bernhard ; Toborek, Michal ; Joshi-Barve, Swati ; Barger, Steven W. ; Barve, Shirish ; Mattson, Mark P. ; McClain, Craig J. / Linoleic acid activates nuclear transcription factor-κB (NF-κB) and induces NF-κB-dependent transcription in cultured endothelial cells. In: American Journal of Clinical Nutrition. 1996 ; Vol. 63, No. 3. pp. 322-328.
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