TY - JOUR
T1 - Linking form to function
T2 - Biophysical aspects of artificial antigen presenting cell design
AU - Perica, Karlo
AU - Kosmides, Alyssa K.
AU - Schneck, Jonathan P.
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Artificial antigen presenting cells (aAPCs) are engineered platforms for T cell activation and expansion, synthesized by coupling T cell activating proteins to the surface of cell lines or biocompatible particles. They can serve both as model systems to study the basic aspects of T cell signaling and translationally as novel approaches for either active or adoptive immunotherapy. Historically, these reductionist systems have not been designed to mimic the temporally and spatially complex interactions observed during endogenous T cell-APC contact, which include receptor organization at both micro- and nanoscales and dynamic changes in cell and membrane morphologies. Here, we review how particle size and shape, as well as heterogenous distribution of T cell activating proteins on the particle surface, are critical aspects of aAPC design. In doing so, we demonstrate how insights derived from endogenous T cell activation can be applied to optimize aAPC, and in turn how aAPC platforms can be used to better understand endogenous T cell stimulation. This article is part of a Special Issue entitled: Nanoscale membrane organisation and signalling.
AB - Artificial antigen presenting cells (aAPCs) are engineered platforms for T cell activation and expansion, synthesized by coupling T cell activating proteins to the surface of cell lines or biocompatible particles. They can serve both as model systems to study the basic aspects of T cell signaling and translationally as novel approaches for either active or adoptive immunotherapy. Historically, these reductionist systems have not been designed to mimic the temporally and spatially complex interactions observed during endogenous T cell-APC contact, which include receptor organization at both micro- and nanoscales and dynamic changes in cell and membrane morphologies. Here, we review how particle size and shape, as well as heterogenous distribution of T cell activating proteins on the particle surface, are critical aspects of aAPC design. In doing so, we demonstrate how insights derived from endogenous T cell activation can be applied to optimize aAPC, and in turn how aAPC platforms can be used to better understand endogenous T cell stimulation. This article is part of a Special Issue entitled: Nanoscale membrane organisation and signalling.
KW - Artificial antigen presenting cell
KW - Immunotherapy
KW - Microparticle
KW - Microscale interaction
KW - Nanoscale interaction
UR - http://www.scopus.com/inward/record.url?scp=84923169786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923169786&partnerID=8YFLogxK
U2 - 10.1016/j.bbamcr.2014.09.001
DO - 10.1016/j.bbamcr.2014.09.001
M3 - Review article
C2 - 25200637
AN - SCOPUS:84923169786
SN - 0167-4889
VL - 1853
SP - 781
EP - 790
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 4
ER -