Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat

John G. Lewis, James L. Weber, Michael B. Petersen, Susan A. Slaugenhaupt, Anne Kwitek, Paula E. May, Andrew C. Warren, Aravinda Chakravarti, Stylianos E. Antonarakis

Research output: Contribution to journalArticle

Abstract

A (GT)n repeat within the anonymous DNA sequence D21S156 was shown to be highly polymorphic in DNA from members of the 40 CEPH families. At least 12 alleles of this locus were recognized by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction (PCR) using primers flanking the (GT)n repeat. The polymorphism information content was 0.82. PCR amplification of DNA from somatic cell hybrid lines mapped D21S156 to human chromosome 21 and linkage analysis localized this marker close to the loci ETS2, D21S3, and HMG14 on chromosomal band 21q22.3. This polymorphism is highly informative and can serve as anchor locus for human chromosome 21.

Original languageEnglish (US)
Pages (from-to)400-402
Number of pages3
JournalGenomics
Volume8
Issue number2
DOIs
StatePublished - 1990

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Dinucleotide Repeats
Chromosomes, Human, Pair 21
Chromosome Mapping
Human Chromosomes
Genetic Markers
HMGN1 Protein
Polymerase Chain Reaction
Hybrid Cells
DNA
DNA-Directed DNA Polymerase
Polyacrylamide Gel Electrophoresis
Alleles
Cell Line

ASJC Scopus subject areas

  • Genetics

Cite this

Lewis, J. G., Weber, J. L., Petersen, M. B., Slaugenhaupt, S. A., Kwitek, A., May, P. E., ... Antonarakis, S. E. (1990). Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat. Genomics, 8(2), 400-402. https://doi.org/10.1016/0888-7543(90)90300-J

Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat. / Lewis, John G.; Weber, James L.; Petersen, Michael B.; Slaugenhaupt, Susan A.; Kwitek, Anne; May, Paula E.; Warren, Andrew C.; Chakravarti, Aravinda; Antonarakis, Stylianos E.

In: Genomics, Vol. 8, No. 2, 1990, p. 400-402.

Research output: Contribution to journalArticle

Lewis, JG, Weber, JL, Petersen, MB, Slaugenhaupt, SA, Kwitek, A, May, PE, Warren, AC, Chakravarti, A & Antonarakis, SE 1990, 'Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat', Genomics, vol. 8, no. 2, pp. 400-402. https://doi.org/10.1016/0888-7543(90)90300-J
Lewis, John G. ; Weber, James L. ; Petersen, Michael B. ; Slaugenhaupt, Susan A. ; Kwitek, Anne ; May, Paula E. ; Warren, Andrew C. ; Chakravarti, Aravinda ; Antonarakis, Stylianos E. / Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat. In: Genomics. 1990 ; Vol. 8, No. 2. pp. 400-402.
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