TY - JOUR
T1 - Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat
AU - Lewis, John G.
AU - Weber, James L.
AU - Petersen, Michael B.
AU - Slaugenhaupt, Susan A.
AU - Kwitek, Anne
AU - May, Paula E.
AU - Warren, Andrew C.
AU - Chakravarti, Aravinda
AU - Antonarakis, Stylianos E.
N1 - Funding Information:
J.L.W. was supported by the Marshfield Clinic and NIH Grant GM41773; M.B.P. by the Danish Research Council and Academy, Else og Mogens Wedell-Wedellsborgs Fond, and a Fulbright Fellowship; A.C. by NIH Grants GM33771 and Research Career Development Award HDOO774; A.C.W. by the Alzheimer’s Association; and S.E.A. and A.C. by NIH Grant PO1 HD24605.
PY - 1990/10
Y1 - 1990/10
N2 - A (GT)n repeat within the anonymous DNA sequence D21S156 was shown to be highly polymorphic in DNA from members of the 40 CEPH families. At least 12 alleles of this locus were recognized by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction (PCR) using primers flanking the (GT)n repeat. The polymorphism information content was 0.82. PCR amplification of DNA from somatic cell hybrid lines mapped D21S156 to human chromosome 21 and linkage analysis localized this marker close to the loci ETS2, D21S3, and HMG14 on chromosomal band 21q22.3. This polymorphism is highly informative and can serve as anchor locus for human chromosome 21.
AB - A (GT)n repeat within the anonymous DNA sequence D21S156 was shown to be highly polymorphic in DNA from members of the 40 CEPH families. At least 12 alleles of this locus were recognized by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction (PCR) using primers flanking the (GT)n repeat. The polymorphism information content was 0.82. PCR amplification of DNA from somatic cell hybrid lines mapped D21S156 to human chromosome 21 and linkage analysis localized this marker close to the loci ETS2, D21S3, and HMG14 on chromosomal band 21q22.3. This polymorphism is highly informative and can serve as anchor locus for human chromosome 21.
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U2 - 10.1016/0888-7543(90)90300-J
DO - 10.1016/0888-7543(90)90300-J
M3 - Article
C2 - 1979059
AN - SCOPUS:0025092562
SN - 0888-7543
VL - 8
SP - 400
EP - 402
JO - Genomics
JF - Genomics
IS - 2
ER -