TY - JOUR
T1 - Linezolid resistance in Mycobacterium tuberculosis isolates at a tertiary care centre in Mumbai, India
AU - Nambiar, Remya
AU - Tornheim, Jeffrey
AU - Diricks, Margo
AU - De Bruyne, Katrien
AU - Sadani, Meeta
AU - Shetty, Anjali
AU - Rodrigues, Camilla
N1 - Funding Information:
supported by bioMérieux India, New Delhi, India. The second author (JAT) was supported by the United States National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (grants K23AI135102 and R21AI122922), the Johns Hopkins University School of Medicine Clinician Scientist Career Development Award, and the NIH Office of the Director, Fogarty International Center, Office of AIDS Research, through the Fogarty Global Health Fellows Program Consortium (R25TW009340).
Publisher Copyright:
© 2021 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2021/7
Y1 - 2021/7
N2 - Background & objectives: Linezolid (LZD) is increasingly being used in tuberculosis (TB) treatment. However, LZD resistance has already been reported, which is highly alarming, given its critical therapeutic role. This study was aimed to phenotypically and genotypically assess LZD resistance in Mycobacterium tuberculosis (MTB) isolates at a laboratory in a tertiary care centre in Mumbai, India. Methods: A sample of 32 consecutive LZD-resistant MTB isolates identified by liquid culture susceptibility testing was subjected to whole-genome sequencing (WGS) on the Illumina NextSeq platform. Sequences were analyzed using BioNumerics software to predict resistance for 12 antibiotics within 15 min. Results: Sixty eight of the 2179 isolates tested for LZD resistance by MGIT-based susceptibility testing (June 2015 to June 2016) were LZD-resistant. Thirty two consecutive LZD-resistant isolates were analyzed by WGS to screen for known mutations conferring LZD resistance. WGS of 32 phenotypically LZD-resistant isolates showed that C154R in the rplC gene and G2814T in the rrl gene were the major resistance determinants. Interpretation & conclusions: LZD resistance poses an important risk to the success of treatment regimens, especially those designed for resistant isolates; such regimens are extensively used in India. As LZD-containing regimens increase in prominence, it is important to support clinical decision-making with an improved understanding of the common mutations conferring LZD resistance and their frequency in different settings.
AB - Background & objectives: Linezolid (LZD) is increasingly being used in tuberculosis (TB) treatment. However, LZD resistance has already been reported, which is highly alarming, given its critical therapeutic role. This study was aimed to phenotypically and genotypically assess LZD resistance in Mycobacterium tuberculosis (MTB) isolates at a laboratory in a tertiary care centre in Mumbai, India. Methods: A sample of 32 consecutive LZD-resistant MTB isolates identified by liquid culture susceptibility testing was subjected to whole-genome sequencing (WGS) on the Illumina NextSeq platform. Sequences were analyzed using BioNumerics software to predict resistance for 12 antibiotics within 15 min. Results: Sixty eight of the 2179 isolates tested for LZD resistance by MGIT-based susceptibility testing (June 2015 to June 2016) were LZD-resistant. Thirty two consecutive LZD-resistant isolates were analyzed by WGS to screen for known mutations conferring LZD resistance. WGS of 32 phenotypically LZD-resistant isolates showed that C154R in the rplC gene and G2814T in the rrl gene were the major resistance determinants. Interpretation & conclusions: LZD resistance poses an important risk to the success of treatment regimens, especially those designed for resistant isolates; such regimens are extensively used in India. As LZD-containing regimens increase in prominence, it is important to support clinical decision-making with an improved understanding of the common mutations conferring LZD resistance and their frequency in different settings.
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U2 - 10.4103/ijmr.IJMR-1168-19
DO - 10.4103/ijmr.IJMR-1168-19
M3 - Article
C2 - 34782533
AN - SCOPUS:85120328611
SN - 0971-5916
VL - 154
SP - 85
EP - 89
JO - Indian Journal of Medical Research
JF - Indian Journal of Medical Research
IS - 1
ER -