Linear-programmed thermal degradation methane chemical-ionization mass spectrometry. I. Peptidoglycan, cell walls, and related compounds from Bacillus

Jerrold A. Adkins; Terence H. Risby; John J. Scocca; Ronald E. Yasbin; John W. Ezzell

doi:10.1016/0165-2370(84)80038-8

Linear-programmed thermal degradation methane chemical-ionization mass spectrometry. I. Peptidoglycan, cell walls, and related compounds from Bacillus

Jerrold A. Adkins, Terence H. Risby, John J. Scocca, Ronald E. Yasbin, John W. Ezzell

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Abstract

Linear-programmed thermal degradation-mass spectrometry was used to study cell wall preparations from gram-positive bacteria of the genus Bacillus and related compounds. Also, a group of strains of Bacillus subtilis grown under various culturing conditions were compared. The temperature-resolved pyrolysis-mass spectra collected for each sample were analyzed. Many ions present in the pyrolysis-mass spectra of large complex biopolymers were found to have the same nominal masses to those found in spectra of related simpler compounds. Results indicate the importance of culture conditions if pyrolysis-mass spectrometry is used to study bacteria. Also, the pyrolysis-mass spectra of cell wall preparations from Bacillus are dominated by aminosugars.

Original language	English (US)
Pages (from-to)	15-33
Number of pages	19
Journal	Journal of Analytical and Applied Pyrolysis
Volume	7
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-2370(84)80038-8
State	Published - Nov 1984

ASJC Scopus subject areas

Analytical Chemistry
Fuel Technology

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@article{a901b944be034a9b8ab599a706a5e0bf,

title = "Linear-programmed thermal degradation methane chemical-ionization mass spectrometry. I. Peptidoglycan, cell walls, and related compounds from Bacillus",

abstract = "Linear-programmed thermal degradation-mass spectrometry was used to study cell wall preparations from gram-positive bacteria of the genus Bacillus and related compounds. Also, a group of strains of Bacillus subtilis grown under various culturing conditions were compared. The temperature-resolved pyrolysis-mass spectra collected for each sample were analyzed. Many ions present in the pyrolysis-mass spectra of large complex biopolymers were found to have the same nominal masses to those found in spectra of related simpler compounds. Results indicate the importance of culture conditions if pyrolysis-mass spectrometry is used to study bacteria. Also, the pyrolysis-mass spectra of cell wall preparations from Bacillus are dominated by aminosugars.",

author = "Adkins, {Jerrold A.} and Risby, {Terence H.} and Scocca, {John J.} and Yasbin, {Ronald E.} and Ezzell, {John W.}",

note = "Funding Information: This work was supported by a grant from the National Institute of Allergy and Infectious Diseases (AI 16384). The MODCOMP II/25 computer system was purchased with funds from the U.S. Environmental Protection Agency (R-806558), the National Institute of Allergy and Infectious Diseases (AI 16384), and the Division of Research Resources at the National Institutes of Health (RR-05445). We also wish to thank M.H. Bozorgzadeh and Tatyana Frenkel for their help in obtaining data.",

year = "1984",

month = nov,

doi = "10.1016/0165-2370(84)80038-8",

language = "English (US)",

volume = "7",

pages = "15--33",

journal = "Journal of Analytical and Applied Pyrolysis",

issn = "0165-2370",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Linear-programmed thermal degradation methane chemical-ionization mass spectrometry. I. Peptidoglycan, cell walls, and related compounds from Bacillus

AU - Adkins, Jerrold A.

AU - Risby, Terence H.

AU - Scocca, John J.

AU - Yasbin, Ronald E.

AU - Ezzell, John W.

N1 - Funding Information: This work was supported by a grant from the National Institute of Allergy and Infectious Diseases (AI 16384). The MODCOMP II/25 computer system was purchased with funds from the U.S. Environmental Protection Agency (R-806558), the National Institute of Allergy and Infectious Diseases (AI 16384), and the Division of Research Resources at the National Institutes of Health (RR-05445). We also wish to thank M.H. Bozorgzadeh and Tatyana Frenkel for their help in obtaining data.

PY - 1984/11

Y1 - 1984/11

N2 - Linear-programmed thermal degradation-mass spectrometry was used to study cell wall preparations from gram-positive bacteria of the genus Bacillus and related compounds. Also, a group of strains of Bacillus subtilis grown under various culturing conditions were compared. The temperature-resolved pyrolysis-mass spectra collected for each sample were analyzed. Many ions present in the pyrolysis-mass spectra of large complex biopolymers were found to have the same nominal masses to those found in spectra of related simpler compounds. Results indicate the importance of culture conditions if pyrolysis-mass spectrometry is used to study bacteria. Also, the pyrolysis-mass spectra of cell wall preparations from Bacillus are dominated by aminosugars.

AB - Linear-programmed thermal degradation-mass spectrometry was used to study cell wall preparations from gram-positive bacteria of the genus Bacillus and related compounds. Also, a group of strains of Bacillus subtilis grown under various culturing conditions were compared. The temperature-resolved pyrolysis-mass spectra collected for each sample were analyzed. Many ions present in the pyrolysis-mass spectra of large complex biopolymers were found to have the same nominal masses to those found in spectra of related simpler compounds. Results indicate the importance of culture conditions if pyrolysis-mass spectrometry is used to study bacteria. Also, the pyrolysis-mass spectra of cell wall preparations from Bacillus are dominated by aminosugars.

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DO - 10.1016/0165-2370(84)80038-8

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AN - SCOPUS:0021522226

SN - 0165-2370

VL - 7

SP - 15

EP - 33

JO - Journal of Analytical and Applied Pyrolysis

JF - Journal of Analytical and Applied Pyrolysis

IS - 1-2

ER -

Linear-programmed thermal degradation methane chemical-ionization mass spectrometry. I. Peptidoglycan, cell walls, and related compounds from Bacillus

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