Linear-programmed thermal degradation methane chemical-ionization mass spectrometry. I. Peptidoglycan, cell walls, and related compounds from Bacillus

Jerrold A. Adkins, Terence H. Risby, John J. Scocca, Ronald E. Yasbin, John W. Ezzell

Research output: Contribution to journalArticlepeer-review

Abstract

Linear-programmed thermal degradation-mass spectrometry was used to study cell wall preparations from gram-positive bacteria of the genus Bacillus and related compounds. Also, a group of strains of Bacillus subtilis grown under various culturing conditions were compared. The temperature-resolved pyrolysis-mass spectra collected for each sample were analyzed. Many ions present in the pyrolysis-mass spectra of large complex biopolymers were found to have the same nominal masses to those found in spectra of related simpler compounds. Results indicate the importance of culture conditions if pyrolysis-mass spectrometry is used to study bacteria. Also, the pyrolysis-mass spectra of cell wall preparations from Bacillus are dominated by aminosugars.

Original languageEnglish (US)
Pages (from-to)15-33
Number of pages19
JournalJournal of Analytical and Applied Pyrolysis
Volume7
Issue number1-2
DOIs
StatePublished - Nov 1984

ASJC Scopus subject areas

  • Analytical Chemistry
  • Fuel Technology

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