Linear immunofluorescence staining of the cutaneous basement membrane zone produced by pemphigoid antibodies: The result of hemidesmosome staining

Diya F. Mutasim, Grant J. Anhalt, Luis A. Diaz, Harish P. Patel

Research output: Contribution to journalArticle

Abstract

Bullous pemphigoid autoantibodies bind the basement membrane zone of stratified squamous epithelium in a linear pattern, as shown by indirect immunofluorescence; however, other patterns of staining, such as tubular (with convolutions), cytoplasmic, and membranous, have been noted. Recently, by using indirect immunoelectron microscopy, we have shown that bullous pemphigoid antibodies bind specifically to hemidesmosomes. The purpose of this investigation was to correlate the various patterns of basement membrane zone staining by bullous pemphigoid antibodies by immunofluorescence and to correlate these patterns with the ultrastructural findings. We employed adult human skin, neonatal human foreskin, neonatal mouse skin, and rabbit cornea as substrates for electron microscopy and indirect immunofluorescence using bullous pemphigoid serum. For indirect immunofluorescence, cryosections were obtained at vertical, oblique, and horizontal planes with respect to the basement membrane zone. We show that the immunofluorescence band of basement membrane zone staining results from the coalescence of fluorescence from individual hemidesmosomes. We also show that the pattern of basement membrane zone staining depends on the ultrastructural morphology of the basement membrane zone in each tissue and on the angle of sectioning.

Original languageEnglish (US)
Pages (from-to)75-82
Number of pages8
JournalJournal of the American Academy of Dermatology
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Dermatology

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