TY - JOUR
T1 - Linear immunofluorescence staining of the cutaneous basement membrane zone produced by pemphigoid antibodies
T2 - The result of hemidesmosome staining
AU - Mutasim, Diya F.
AU - Anhalt, Grant J.
AU - Diaz, Luis A.
AU - Patel, Harish P.
N1 - Funding Information:
From the Immunodermatology Unit, Department of Dermatology, The Johns Hopkins University. Supported in part by U.S. Public Health Service Grant Nos. RO1-AM 32081, RO1-AM 32599, RO1-AM 33625, R23-AM 32079, and R23-AM 32490, from the National Institutes of Health, and Nltt Training Grant No. 5T32 AM 07324. Accepted for publication July 1, 1986. Reprint requests to: Dr. Diya F. Mutasim, Department of Dermatol-ogy, The Johns Hopkins University, 909G Blaloek Bldg., 600 N. Wolfe St., Baltimore, MD 21205.
PY - 1987
Y1 - 1987
N2 - Bullous pemphigoid autoantibodies bind the basement membrane zone of stratified squamous epithelium in a linear pattern, as shown by indirect immunofluorescence; however, other patterns of staining, such as tubular (with convolutions), cytoplasmic, and membranous, have been noted. Recently, by using indirect immunoelectron microscopy, we have shown that bullous pemphigoid antibodies bind specifically to hemidesmosomes. The purpose of this investigation was to correlate the various patterns of basement membrane zone staining by bullous pemphigoid antibodies by immunofluorescence and to correlate these patterns with the ultrastructural findings. We employed adult human skin, neonatal human foreskin, neonatal mouse skin, and rabbit cornea as substrates for electron microscopy and indirect immunofluorescence using bullous pemphigoid serum. For indirect immunofluorescence, cryosections were obtained at vertical, oblique, and horizontal planes with respect to the basement membrane zone. We show that the immunofluorescence band of basement membrane zone staining results from the coalescence of fluorescence from individual hemidesmosomes. We also show that the pattern of basement membrane zone staining depends on the ultrastructural morphology of the basement membrane zone in each tissue and on the angle of sectioning.
AB - Bullous pemphigoid autoantibodies bind the basement membrane zone of stratified squamous epithelium in a linear pattern, as shown by indirect immunofluorescence; however, other patterns of staining, such as tubular (with convolutions), cytoplasmic, and membranous, have been noted. Recently, by using indirect immunoelectron microscopy, we have shown that bullous pemphigoid antibodies bind specifically to hemidesmosomes. The purpose of this investigation was to correlate the various patterns of basement membrane zone staining by bullous pemphigoid antibodies by immunofluorescence and to correlate these patterns with the ultrastructural findings. We employed adult human skin, neonatal human foreskin, neonatal mouse skin, and rabbit cornea as substrates for electron microscopy and indirect immunofluorescence using bullous pemphigoid serum. For indirect immunofluorescence, cryosections were obtained at vertical, oblique, and horizontal planes with respect to the basement membrane zone. We show that the immunofluorescence band of basement membrane zone staining results from the coalescence of fluorescence from individual hemidesmosomes. We also show that the pattern of basement membrane zone staining depends on the ultrastructural morphology of the basement membrane zone in each tissue and on the angle of sectioning.
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U2 - 10.1016/S0190-9622(87)70006-1
DO - 10.1016/S0190-9622(87)70006-1
M3 - Article
C2 - 2433316
AN - SCOPUS:0023106233
SN - 0190-9622
VL - 16
SP - 75
EP - 82
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
IS - 1
ER -