Lineage Tracing Using Cux2-Cre and Cux2-CreERT2 Mice

Cristina Gil-Sanz, Ana Espinosa, Santiago P. Fregoso, Krista K. Bluske, Christopher L. Cunningham, Isabel Martinez-Garay, Hongkui Zeng, Santos J. Franco, Ulrich Müller

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Using genetic fate-mapping with Cux2-Cre and Cux2-CreERT2 mice we demonstrated that the neocorticalventricular zone (VZ) contains radial glial cells (RGCs) with restricted fate potentials (Franco etal., 2012). Using the same mouse lines, Guo etal. (2013) concluded that the neocortical VZ does not contain lineage-restricted RGCs. We now show that the recombination pattern in Cux2-Cre/CreERT2 mice depends on genetic background and breeding strategies. We provide evidence that Guo etal. likely reached different conclusions because they worked with transgenic sublines with drifted transgene expression patterns. In Cux2-Cre and Cux2-CreERT2 mice that recapitulate the endogenous Cux2 expression pattern, the vast majority of fate-mapped neurons express Satb2 but not Ctip2, confirming that a restricted subset of all neocortical projection neurons belongs to the Cux2 lineage. This Matters Arising paper is in response to Guo etal. (2013), published in Neuron. See also the Matters Arising Response paper by Eckler etal. (2015), published concurrently with this Matters Arising in Neuron.

Original languageEnglish (US)
Pages (from-to)1091-1099
Number of pages9
Issue number4
StatePublished - May 20 2015
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)


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