Lineage commitment and maturation induction in normal and leukemic preprogenitor cells

D. Metcalf, Y. Laâbi, M. Moore, P. Lansdorp, S. Sharkis, G. Keller, B. Löwenberg, J. E. Dick

Research output: Contribution to journalArticle

Abstract

Analysis of the critical cellular processes of self-generation, commitment, and maturation induction ideally requires the use of clonal cultures using cells with a capacity to undergo allthree processes. Preprogenitor cells from normal mouse marrow are proving useful cells for such studies. Cells of a newly established cloned leukemic cell line, the GB2, are providing a useful analogous leukemic system because GB2 cells form stratified subpopulations of clonogenic cells able to be clonally analyzed in vitro in which self-renewal is demonstrable, but in which near-normal maturation can be induced by a wide range of hematopoietic regulators.

Original languageEnglish (US)
Pages (from-to)278-292
Number of pages15
JournalAnnals of the New York Academy of Sciences
Volume938
DOIs
StatePublished - 2001

Keywords

  • Blast colonies
  • Commitment
  • Cytokines
  • Hematopoiesis
  • Leukemia
  • Maturation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Metcalf, D., Laâbi, Y., Moore, M., Lansdorp, P., Sharkis, S., Keller, G., Löwenberg, B., & Dick, J. E. (2001). Lineage commitment and maturation induction in normal and leukemic preprogenitor cells. Annals of the New York Academy of Sciences, 938, 278-292. https://doi.org/10.1111/j.1749-6632.2001.tb03597.x