LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk

S. M. Tajuddin, A. F S Amaral, A. F. Fernández, S. Chanock, D. T. Silverman, A. Tardón, A. Carrato, M. García-Closas, B. P. Jackson, E. G. Toraño, M. Márquez, R. G. Urdinguio, R. García-Closas, N. Rothman, M. Kogevinas, F. X. Real, M. F. Fraga, N. Malats

Research output: Contribution to journalArticle

Abstract

Background:Aberrant global DNA methylation is shown to increase cancer risk. LINE-1 has been proven a measure of global DNA methylation. The objectives of this study were to assess the association between LINE-1 methylation level and bladder cancer risk and to evaluate effect modification by environmental and genetic factors.Methods:Bisulphite-treated leukocyte DNA from 952 cases and 892 hospital controls was used to measure LINE-1 methylation level at four CpG sites by pyrosequencing. Logistic regression model was fitted to estimate odds ratios (ORs) and 95% confidence intervals (95% CIs). Interactions between LINE-1 methylation levels and environmental and genetic factors were assessed.Results:The risk of bladder cancer followed a nonlinear association with LINE-1 methylation. Compared with subjects in the middle tertile, the adjusted OR for subjects in the lower and the higher tertiles were 1.26 (95% CI 0.99-1.60, P=0.06) and 1.33 (95% CI 1.05-1.69, P=0.02), respectively. This association significantly increased among individuals homozygous for the major allele of five single-nucleotide polymorphisms located in the phosphatidylethanolamine N-methyltransferase gene (corrected P-interaction

Original languageEnglish (US)
Pages (from-to)2123-2130
Number of pages8
JournalBritish Journal of Cancer
Volume110
Issue number8
DOIs
StatePublished - Apr 15 2014
Externally publishedYes

Fingerprint

Phosphatidylethanolamine N-Methyltransferase
Urinary Bladder Neoplasms
Methylation
Leukocytes
DNA
DNA Methylation
Confidence Intervals
Logistic Models
Odds Ratio
Single Nucleotide Polymorphism
Alleles
Genes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tajuddin, S. M., Amaral, A. F. S., Fernández, A. F., Chanock, S., Silverman, D. T., Tardón, A., ... Malats, N. (2014). LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk. British Journal of Cancer, 110(8), 2123-2130. https://doi.org/10.1038/bjc.2014.67

LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk. / Tajuddin, S. M.; Amaral, A. F S; Fernández, A. F.; Chanock, S.; Silverman, D. T.; Tardón, A.; Carrato, A.; García-Closas, M.; Jackson, B. P.; Toraño, E. G.; Márquez, M.; Urdinguio, R. G.; García-Closas, R.; Rothman, N.; Kogevinas, M.; Real, F. X.; Fraga, M. F.; Malats, N.

In: British Journal of Cancer, Vol. 110, No. 8, 15.04.2014, p. 2123-2130.

Research output: Contribution to journalArticle

Tajuddin, SM, Amaral, AFS, Fernández, AF, Chanock, S, Silverman, DT, Tardón, A, Carrato, A, García-Closas, M, Jackson, BP, Toraño, EG, Márquez, M, Urdinguio, RG, García-Closas, R, Rothman, N, Kogevinas, M, Real, FX, Fraga, MF & Malats, N 2014, 'LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk', British Journal of Cancer, vol. 110, no. 8, pp. 2123-2130. https://doi.org/10.1038/bjc.2014.67
Tajuddin, S. M. ; Amaral, A. F S ; Fernández, A. F. ; Chanock, S. ; Silverman, D. T. ; Tardón, A. ; Carrato, A. ; García-Closas, M. ; Jackson, B. P. ; Toraño, E. G. ; Márquez, M. ; Urdinguio, R. G. ; García-Closas, R. ; Rothman, N. ; Kogevinas, M. ; Real, F. X. ; Fraga, M. F. ; Malats, N. / LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk. In: British Journal of Cancer. 2014 ; Vol. 110, No. 8. pp. 2123-2130.
@article{6d8e6584d58546f6a6be1632c0e49ab9,
title = "LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk",
abstract = "Background:Aberrant global DNA methylation is shown to increase cancer risk. LINE-1 has been proven a measure of global DNA methylation. The objectives of this study were to assess the association between LINE-1 methylation level and bladder cancer risk and to evaluate effect modification by environmental and genetic factors.Methods:Bisulphite-treated leukocyte DNA from 952 cases and 892 hospital controls was used to measure LINE-1 methylation level at four CpG sites by pyrosequencing. Logistic regression model was fitted to estimate odds ratios (ORs) and 95{\%} confidence intervals (95{\%} CIs). Interactions between LINE-1 methylation levels and environmental and genetic factors were assessed.Results:The risk of bladder cancer followed a nonlinear association with LINE-1 methylation. Compared with subjects in the middle tertile, the adjusted OR for subjects in the lower and the higher tertiles were 1.26 (95{\%} CI 0.99-1.60, P=0.06) and 1.33 (95{\%} CI 1.05-1.69, P=0.02), respectively. This association significantly increased among individuals homozygous for the major allele of five single-nucleotide polymorphisms located in the phosphatidylethanolamine N-methyltransferase gene (corrected P-interaction",
author = "Tajuddin, {S. M.} and Amaral, {A. F S} and Fern{\'a}ndez, {A. F.} and S. Chanock and Silverman, {D. T.} and A. Tard{\'o}n and A. Carrato and M. Garc{\'i}a-Closas and Jackson, {B. P.} and Tora{\~n}o, {E. G.} and M. M{\'a}rquez and Urdinguio, {R. G.} and R. Garc{\'i}a-Closas and N. Rothman and M. Kogevinas and Real, {F. X.} and Fraga, {M. F.} and N. Malats",
year = "2014",
month = "4",
day = "15",
doi = "10.1038/bjc.2014.67",
language = "English (US)",
volume = "110",
pages = "2123--2130",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk

AU - Tajuddin, S. M.

AU - Amaral, A. F S

AU - Fernández, A. F.

AU - Chanock, S.

AU - Silverman, D. T.

AU - Tardón, A.

AU - Carrato, A.

AU - García-Closas, M.

AU - Jackson, B. P.

AU - Toraño, E. G.

AU - Márquez, M.

AU - Urdinguio, R. G.

AU - García-Closas, R.

AU - Rothman, N.

AU - Kogevinas, M.

AU - Real, F. X.

AU - Fraga, M. F.

AU - Malats, N.

PY - 2014/4/15

Y1 - 2014/4/15

N2 - Background:Aberrant global DNA methylation is shown to increase cancer risk. LINE-1 has been proven a measure of global DNA methylation. The objectives of this study were to assess the association between LINE-1 methylation level and bladder cancer risk and to evaluate effect modification by environmental and genetic factors.Methods:Bisulphite-treated leukocyte DNA from 952 cases and 892 hospital controls was used to measure LINE-1 methylation level at four CpG sites by pyrosequencing. Logistic regression model was fitted to estimate odds ratios (ORs) and 95% confidence intervals (95% CIs). Interactions between LINE-1 methylation levels and environmental and genetic factors were assessed.Results:The risk of bladder cancer followed a nonlinear association with LINE-1 methylation. Compared with subjects in the middle tertile, the adjusted OR for subjects in the lower and the higher tertiles were 1.26 (95% CI 0.99-1.60, P=0.06) and 1.33 (95% CI 1.05-1.69, P=0.02), respectively. This association significantly increased among individuals homozygous for the major allele of five single-nucleotide polymorphisms located in the phosphatidylethanolamine N-methyltransferase gene (corrected P-interaction

AB - Background:Aberrant global DNA methylation is shown to increase cancer risk. LINE-1 has been proven a measure of global DNA methylation. The objectives of this study were to assess the association between LINE-1 methylation level and bladder cancer risk and to evaluate effect modification by environmental and genetic factors.Methods:Bisulphite-treated leukocyte DNA from 952 cases and 892 hospital controls was used to measure LINE-1 methylation level at four CpG sites by pyrosequencing. Logistic regression model was fitted to estimate odds ratios (ORs) and 95% confidence intervals (95% CIs). Interactions between LINE-1 methylation levels and environmental and genetic factors were assessed.Results:The risk of bladder cancer followed a nonlinear association with LINE-1 methylation. Compared with subjects in the middle tertile, the adjusted OR for subjects in the lower and the higher tertiles were 1.26 (95% CI 0.99-1.60, P=0.06) and 1.33 (95% CI 1.05-1.69, P=0.02), respectively. This association significantly increased among individuals homozygous for the major allele of five single-nucleotide polymorphisms located in the phosphatidylethanolamine N-methyltransferase gene (corrected P-interaction

UR - http://www.scopus.com/inward/record.url?scp=84901953680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901953680&partnerID=8YFLogxK

U2 - 10.1038/bjc.2014.67

DO - 10.1038/bjc.2014.67

M3 - Article

VL - 110

SP - 2123

EP - 2130

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 8

ER -