Light-triggered proton movements in retinal discs from the frog

Peter C. Maloney, Dominic M K Lam, T. Hastings Wilson

Research output: Contribution to journalArticle

Abstract

Illumination of retinal discs from dark adapted frogs caused a rapid and transient alkalinization of the suspension medium, followed by a slower and more prolonged acidification. Pretreatment with valinomycin, gramicidin or a proton conductor did not change this pattern, but the initial proton uptake was prevented by concentrations of a detergent or alcohol that had no effect on the acidification process. The initial transient proton uptake corresponded to a minimum of 5.7 H+ taken up per rhodopsin bleached, with an elevation of this stoichiometry at early times of illumination. It is suggested that bleaching of rhodopsin contained in retinal discs initiates a transmembrane ion flux that is reflected by a net proton entry.

Original languageEnglish (US)
Pages (from-to)420-426
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume93
Issue number2
DOIs
StatePublished - Mar 28 1980

Fingerprint

Anura
Protons
Light
Rhodopsin
Acidification
Lighting
Valinomycin
Gramicidin
Bleaching
Stoichiometry
Detergents
Suspensions
Alcohols
Ions
Fluxes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Light-triggered proton movements in retinal discs from the frog. / Maloney, Peter C.; Lam, Dominic M K; Wilson, T. Hastings.

In: Biochemical and Biophysical Research Communications, Vol. 93, No. 2, 28.03.1980, p. 420-426.

Research output: Contribution to journalArticle

Maloney, Peter C. ; Lam, Dominic M K ; Wilson, T. Hastings. / Light-triggered proton movements in retinal discs from the frog. In: Biochemical and Biophysical Research Communications. 1980 ; Vol. 93, No. 2. pp. 420-426.
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