Light scatter spectroscopy and imaging of cellular and subcellular events

Nada N. Boustany, Nitish V. Thakor

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Thin ex vivo biological specimens, such as single cells and tissue slices, are widely used in biology and medicine. Optical microscopic imaging and spectroscopic techniques are ideal tools for probing the composition of these specimens nondestructively and with minimal sample preparation and handling. This chapter focuses on elastic scattering as one of these optically based analytical techniques. Unlike most turbid tissues analyzed in vivo, light scattering in these relatively thin ex vivo specimens is dominated by single scattering. Here, we describe the methods currently used to collect and interpret elastic scattering data from thin biological specimens, and biomedical applications in this field.

Original languageEnglish (US)
Title of host publicationBiomedical Photonics
Subtitle of host publicationHandbook
PublisherCRC Press
Pages16-1-16-23
ISBN (Electronic)9780203008997
ISBN (Print)0849311160, 9780849311161
StatePublished - Jan 1 2003

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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