Light-dependent spin trapping of hydroxyl radical from human erythrocytes

Leon A. Bynoe, Sovitj Pou, John D. Gottsch, Gerald M. Rosen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The generation of reactive oxygen species from human erythrocytes has previously been demonstrated. Furthermore, erythrocytic protoporphyrin IX has been shown to generate superoxide and singlet oxygen when exposed to light. These findings suggest that a component of erythrocytic reactive oxygen species production may be light-dependent. By inhibiting erythrocyte superoxide dismutase, catalase, and glutathione peroxidase with N,N-diethyldithiocarbamate or sodium cyanide, we demonstrate the light-dependent generation of hydroxyl radical in human erythrocytes using spin trapping/Electron Spin Resonance spectroscopy. This finding may be significant in tissues where blood is exposed to light, such as in the eye.

Original languageEnglish (US)
Pages (from-to)1305-1310
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Sep 30 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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