Light and life in baltimore - And beyond

Research output: Contribution to journalArticle

Abstract

Baltimore has been the home of numerous biophysical studies using light to probe cells. One such study, quantitative measurement of lateral diffusion of rhodopsin, set the standard for experiments in which recovery after photobleaching is used to measure lateral diffusion. Development of this method from specialized microscopes to commercial scanning confocal microscopes has led to widespread use of the technique to measure lateral diffusion of membrane proteins and lipids, and as well diffusion and binding interactions in cell organelles and cytoplasm. Perturbation of equilibrium distributions by photobleaching has also been developed into a robust method to image molecular proximity in terms of fluorescence resonance energy transfer between donor and acceptor fluorophores.

Original languageEnglish (US)
Pages (from-to)466-470
Number of pages5
JournalBiophysical Journal
Volume108
Issue number3
DOIs
StatePublished - Feb 3 2015

Fingerprint

Baltimore
Photobleaching
Light
Fluorescence Resonance Energy Transfer
Rhodopsin
Membrane Lipids
Cell Communication
Organelles
Membrane Proteins
Cytoplasm

ASJC Scopus subject areas

  • Biophysics

Cite this

Light and life in baltimore - And beyond. / Edidin, Michael A.

In: Biophysical Journal, Vol. 108, No. 3, 03.02.2015, p. 466-470.

Research output: Contribution to journalArticle

@article{174bd9d9caf74162a51e48442fba5751,
title = "Light and life in baltimore - And beyond",
abstract = "Baltimore has been the home of numerous biophysical studies using light to probe cells. One such study, quantitative measurement of lateral diffusion of rhodopsin, set the standard for experiments in which recovery after photobleaching is used to measure lateral diffusion. Development of this method from specialized microscopes to commercial scanning confocal microscopes has led to widespread use of the technique to measure lateral diffusion of membrane proteins and lipids, and as well diffusion and binding interactions in cell organelles and cytoplasm. Perturbation of equilibrium distributions by photobleaching has also been developed into a robust method to image molecular proximity in terms of fluorescence resonance energy transfer between donor and acceptor fluorophores.",
author = "Edidin, {Michael A}",
year = "2015",
month = "2",
day = "3",
doi = "10.1016/j.bpj.2014.12.012",
language = "English (US)",
volume = "108",
pages = "466--470",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "3",

}

TY - JOUR

T1 - Light and life in baltimore - And beyond

AU - Edidin, Michael A

PY - 2015/2/3

Y1 - 2015/2/3

N2 - Baltimore has been the home of numerous biophysical studies using light to probe cells. One such study, quantitative measurement of lateral diffusion of rhodopsin, set the standard for experiments in which recovery after photobleaching is used to measure lateral diffusion. Development of this method from specialized microscopes to commercial scanning confocal microscopes has led to widespread use of the technique to measure lateral diffusion of membrane proteins and lipids, and as well diffusion and binding interactions in cell organelles and cytoplasm. Perturbation of equilibrium distributions by photobleaching has also been developed into a robust method to image molecular proximity in terms of fluorescence resonance energy transfer between donor and acceptor fluorophores.

AB - Baltimore has been the home of numerous biophysical studies using light to probe cells. One such study, quantitative measurement of lateral diffusion of rhodopsin, set the standard for experiments in which recovery after photobleaching is used to measure lateral diffusion. Development of this method from specialized microscopes to commercial scanning confocal microscopes has led to widespread use of the technique to measure lateral diffusion of membrane proteins and lipids, and as well diffusion and binding interactions in cell organelles and cytoplasm. Perturbation of equilibrium distributions by photobleaching has also been developed into a robust method to image molecular proximity in terms of fluorescence resonance energy transfer between donor and acceptor fluorophores.

UR - http://www.scopus.com/inward/record.url?scp=84947201734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947201734&partnerID=8YFLogxK

U2 - 10.1016/j.bpj.2014.12.012

DO - 10.1016/j.bpj.2014.12.012

M3 - Article

C2 - 25650914

AN - SCOPUS:84947201734

VL - 108

SP - 466

EP - 470

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 3

ER -