Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery

James C. Schaeffer, Chien Liang Lin, Shigeo Kitayama, George R. Uhl

Research output: Contribution to journalArticle

Abstract

A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening in described.

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalMolecular Brain Research
Volume9
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

Keywords

  • cDNA
  • Expression cloning
  • Ligand autoradiographic receptor screening
  • Receptor cloning
  • β-Adrenergic receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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