Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery

James C. Schaeffer; Chien Liang Lin; Shigeo Kitayama; George R. Uhl

doi:10.1016/0169-328X(91)90012-M

Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery

James C. Schaeffer, Chien Liang Lin, Shigeo Kitayama, George R. Uhl

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening in described.

Original language	English (US)
Pages (from-to)	271-276
Number of pages	6
Journal	Molecular Brain Research
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/0169-328X(91)90012-M
State	Published - 1991
Externally published	Yes

Keywords

cDNA
Expression cloning
Ligand autoradiographic receptor screening
Receptor cloning
β-Adrenergic receptor

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(91)90012-M

Cite this

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title = "Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery",

abstract = "A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening in described.",

keywords = "cDNA, Expression cloning, Ligand autoradiographic receptor screening, Receptor cloning, β-Adrenergic receptor",

author = "Schaeffer, {James C.} and Lin, {Chien Liang} and Shigeo Kitayama and Uhl, {George R.}",

year = "1991",

doi = "10.1016/0169-328X(91)90012-M",

language = "English (US)",

volume = "9",

pages = "271--276",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier",

number = "3",

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T1 - Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery

AU - Schaeffer, James C.

AU - Lin, Chien Liang

AU - Kitayama, Shigeo

AU - Uhl, George R.

PY - 1991

Y1 - 1991

N2 - A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening in described.

AB - A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening in described.

KW - cDNA

KW - Expression cloning

KW - Ligand autoradiographic receptor screening

KW - Receptor cloning

KW - β-Adrenergic receptor

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UR - http://www.scopus.com/inward/citedby.url?scp=0026033115&partnerID=8YFLogxK

U2 - 10.1016/0169-328X(91)90012-M

DO - 10.1016/0169-328X(91)90012-M

M3 - Article

C2 - 1851530

AN - SCOPUS:0026033115

SN - 0169-328X

VL - 9

SP - 271

EP - 276

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 3

ER -

Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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