TY - JOUR
T1 - Leukocyte polycyclic aromatic hydrocarbon-DNA adduct formation and colorectal adenoma
AU - Gunter, Marc J.
AU - Divi, Rao L.
AU - Kulldorff, Martin
AU - Vermeulen, Roel
AU - Haverkos, Kathryn J.
AU - Kuo, Maryanne M.
AU - Strickland, Paul
AU - Poirier, Miriam C.
AU - Rothman, Nathaniel
AU - Sinha, Rashmi
N1 - Funding Information:
We are grateful to Jane Curtin of Information Management Systems for assistance in data management for this study, and Jackie King of Bioreliance for sample handling. This work has been supported in part by the Intramural Program of the National Institutes of Health (National Cancer Institute, Center for Cancer Research).
PY - 2007/7
Y1 - 2007/7
N2 - Consumption of charbroiled red meat and meat-derived polycyclic aromatic hydrocarbons (PAHs) has been associated with risk of colorectal adenoma, a precursor of colorectal cancer. Furthermore, leukocyte PAH-DNA adduct levels have been demonstrated to increase in response to charbroiled red meat intake but to date there have been no studies that have investigated the relationship between leukocyte PAH-DNA adduct levels and risk of colorectal adenoma. We investigated the relation of leukocyte PAH-DNA adduct formation and colorectal adenoma in a clinic-based case-control study of colorectal adenomas. The study comprised 82 cases of colorectal adenoma and 111 polyp-free controls, none of whom were current smokers. Leukocyte PAH-DNA adducts were measured by a sensitive chemiluminescence immunoassay using an antiserum elicited against DNA modified with (±)-7b, 8α-dihydroxy-9α,10a-epoxy-7,8,9,10-tetrahydro-benzo[a]pyrene that recognizes several PAHs bound to human DNA. Leukocyte PAH-DNA adduct levels were higher among colorectal adenoma cases (median, 1.4 adducts per 108 nucleotides) than polyp-free controls (median, 1.2 adducts per 108 nucleotides) (P=0.02). There was a positive association between PAH-DNA adduct level and adenoma prevalence: each unit increase in PAH-DNA adduct level (per 108 nucleotides) was associated with an odds ratio (OR) of 1.5 [95% confidence interval (CI), 1.1-2.2]. In addition, a comparison of the lowest quartile for PAH-DNA adduct level with the highest quartile yielded an OR of 2.8 (95% CI, 1.2-6.5; Ptrend = 0.048) for risk of colorectal adenoma. These data support a link between PAH exposure and colorectal adenoma.
AB - Consumption of charbroiled red meat and meat-derived polycyclic aromatic hydrocarbons (PAHs) has been associated with risk of colorectal adenoma, a precursor of colorectal cancer. Furthermore, leukocyte PAH-DNA adduct levels have been demonstrated to increase in response to charbroiled red meat intake but to date there have been no studies that have investigated the relationship between leukocyte PAH-DNA adduct levels and risk of colorectal adenoma. We investigated the relation of leukocyte PAH-DNA adduct formation and colorectal adenoma in a clinic-based case-control study of colorectal adenomas. The study comprised 82 cases of colorectal adenoma and 111 polyp-free controls, none of whom were current smokers. Leukocyte PAH-DNA adducts were measured by a sensitive chemiluminescence immunoassay using an antiserum elicited against DNA modified with (±)-7b, 8α-dihydroxy-9α,10a-epoxy-7,8,9,10-tetrahydro-benzo[a]pyrene that recognizes several PAHs bound to human DNA. Leukocyte PAH-DNA adduct levels were higher among colorectal adenoma cases (median, 1.4 adducts per 108 nucleotides) than polyp-free controls (median, 1.2 adducts per 108 nucleotides) (P=0.02). There was a positive association between PAH-DNA adduct level and adenoma prevalence: each unit increase in PAH-DNA adduct level (per 108 nucleotides) was associated with an odds ratio (OR) of 1.5 [95% confidence interval (CI), 1.1-2.2]. In addition, a comparison of the lowest quartile for PAH-DNA adduct level with the highest quartile yielded an OR of 2.8 (95% CI, 1.2-6.5; Ptrend = 0.048) for risk of colorectal adenoma. These data support a link between PAH exposure and colorectal adenoma.
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U2 - 10.1093/carcin/bgm022
DO - 10.1093/carcin/bgm022
M3 - Article
C2 - 17277232
AN - SCOPUS:34547756959
SN - 0143-3334
VL - 28
SP - 1426
EP - 1429
JO - Carcinogenesis
JF - Carcinogenesis
IS - 7
ER -