Various rat peritoneal cell suspensions were labeled in vitro with 51Cr and then adoptively transferred intravenously into isologous recipients. An analysis of the circulating radioactivity indicated that ≅ 80 per cent was cell-associated. Following the injection of labeled peritoneal cells taken one day after induction of an exudate with casein, only labeled neutrophils were identified in circulation, whereas after injecting cells from 3-day exudates, only labeled nonlymphocytic mononuclear cells were circulating. Accumulation of these cells in an inflammation induced by the subplantar injection of carrageenin was determined by direct scintillation counting of the amputated extremities and employing controls for plasma radioactivity and the specific activity of the labeled cells. There was a time-related accumulation of 51Cr neutrophils (maximum 1 to 5 hours) and mononuclear cells (5 to 18 hours maximum). The methods employed apparently accurately reflected an active and specific process of cell accumulation as evidenced by the following: (1) there was a direct relationship between the numbers of both cell types injected, circulating and accumulating in the inflammation, (2) the kinetics of cell accumulation was cell type-specific, (3) there was no time-related accumulation of either labeled erythrocytes or lymph node lymphocytes which excluded nonspecific particle sequestration, (4) accumulation of labeled leukocytes was temporally unrelated to vascular permeability changes, and (5) the kinetics of cell accumulation quantitatively reproduced histologic events.
|Original language||English (US)|
|Number of pages||16|
|Journal||The Journal of laboratory and clinical medicine|
|State||Published - Sep 1974|
ASJC Scopus subject areas
- Pathology and Forensic Medicine