Leucocyte-associated immunoglobulin-like receptor-1 is an inhibitory regulator of contact hypersensitivity

Ryusuke Omiya, Fumihiko Tsushima, Hidehiko Narazaki, Yukimi Sakoda, Atsuo Kuramasu, Youn Kim, Haiying Xu, Hideto Tamura, Gefeng Zhu, Lieping Chen, Koji Tamada

Research output: Contribution to journalArticle

Abstract

Leucocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is a membrane receptor of the immunoglobulin (Ig) superfamily that is expressed on most types of haematopoietic cells, and delivers inhibitory signals through interacting with collagens. In order to elucidate the immunological functions of LAIR-1 in vivo, we established transgenic mice expressing a chimeric protein composed of the extracellular domain of LAIR-1 fused with an Ig tag (LAIR-1-Ig), which acts as a decoy by competing with endogenous LAIR-1. The transgenic mice showed an increased susceptibility for development of contact hypersensitivity (CHS), an experimental model of allergic contact dermatitis, in association with enhanced hapten-specific T-cell responses. When T cells from the hapten-sensitized donor mice were transferred into non-sensitized recipients, treatment of either donor mice or recipient mice with LAIR-1-Ig protein accelerated CHS, suggesting a potentially negative role of LAIR-1 in both the sensitization and the elicitation of hapten-reactive T cells. In vitro assays revealed that LAIR-1 decreased the production of interleukin-6 and interleukin-12 in dendritic cells, and inhibited the proliferation and cytokine production of naïve and memory T cells along with G0-G1 cell cycle arrest. Collectively, our findings suggest that LAIR-1 plays a crucial inhibitory role in CHS by regulating antigen-presenting cell and T-cell functions.

Original languageEnglish (US)
Pages (from-to)543-555
Number of pages13
JournalImmunology
Volume128
Issue number4
DOIs
StatePublished - Dec 2009

Fingerprint

Contact Dermatitis
Immunoglobulins
Haptens
T-Lymphocytes
Transgenic Mice
G1 Phase Cell Cycle Checkpoints
leukocyte-associated immunoglobulin-like receptor 1
Allergic Contact Dermatitis
Antigen-Presenting Cells
Interleukin-12
Dendritic Cells
Interleukin-6
Proteins
Theoretical Models
Collagen
Cell Proliferation
Cytokines
Membranes

Keywords

  • Co-signal molecule
  • Contact hypersensitivity
  • Dendritic cells
  • LAIR-1
  • T cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Omiya, R., Tsushima, F., Narazaki, H., Sakoda, Y., Kuramasu, A., Kim, Y., ... Tamada, K. (2009). Leucocyte-associated immunoglobulin-like receptor-1 is an inhibitory regulator of contact hypersensitivity. Immunology, 128(4), 543-555. https://doi.org/10.1111/j.1365-2567.2009.03140.x

Leucocyte-associated immunoglobulin-like receptor-1 is an inhibitory regulator of contact hypersensitivity. / Omiya, Ryusuke; Tsushima, Fumihiko; Narazaki, Hidehiko; Sakoda, Yukimi; Kuramasu, Atsuo; Kim, Youn; Xu, Haiying; Tamura, Hideto; Zhu, Gefeng; Chen, Lieping; Tamada, Koji.

In: Immunology, Vol. 128, No. 4, 12.2009, p. 543-555.

Research output: Contribution to journalArticle

Omiya, R, Tsushima, F, Narazaki, H, Sakoda, Y, Kuramasu, A, Kim, Y, Xu, H, Tamura, H, Zhu, G, Chen, L & Tamada, K 2009, 'Leucocyte-associated immunoglobulin-like receptor-1 is an inhibitory regulator of contact hypersensitivity', Immunology, vol. 128, no. 4, pp. 543-555. https://doi.org/10.1111/j.1365-2567.2009.03140.x
Omiya, Ryusuke ; Tsushima, Fumihiko ; Narazaki, Hidehiko ; Sakoda, Yukimi ; Kuramasu, Atsuo ; Kim, Youn ; Xu, Haiying ; Tamura, Hideto ; Zhu, Gefeng ; Chen, Lieping ; Tamada, Koji. / Leucocyte-associated immunoglobulin-like receptor-1 is an inhibitory regulator of contact hypersensitivity. In: Immunology. 2009 ; Vol. 128, No. 4. pp. 543-555.
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