Lethal disruption of the yeast actin gene by integrative DNA transformation

David Shortle, James E. Haber, David Botstein

Research output: Contribution to journalArticlepeer-review

Abstract

A mutant allele of the chromosomal locus corresponding to the cloned actin gene of the yeast Saccharomyces cerevisiae has been constructed by DNA transformation with a hybrid plasmid which integrates into, and thereby disrupts, the protein-encoding sequences of the gene. In a diploid strain of yeast, disruption of the actin gene on one chromosome results in a mutation that segregates as a recessive lethal tightly linked to a selectable genetic marker on the integrated plasmid. The actin gene, therefore, must encode an essential function for yeast cell growth.

Original languageEnglish (US)
Pages (from-to)371-373
Number of pages3
JournalScience
Volume217
Issue number4557
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • General

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