Less repair of pyrimidine dimers and single-strand breaks in genes by Scid cells

Rudaina H. Alrefai, E. Jeffrey Beecham, Vilhelm A. Bohr, Patricia J. Gearhart

Research output: Contribution to journalArticle

Abstract

Severe combined immunodeficient (Scid) mice have a mutation in the catalytic subunit of the DNA binding protein kinase that is involved in repair of double-strand breaks in DNA. To determine if the protein also influences repair of single-strand breaks, we examined the ability of Scid cells to repair lesions introduced by ultraviolet light and γ-ray irradiation. DNA repair was measured both in total genomic DNA and in specific genes from murine Scid and wildtype fibroblast cell lines. The removal of pyrimidine dimers and repair of strand breaks in genes was measured using quantitative Southern blot analyses. After ultraviolet irradiation, there was no significant difference in the repair of photoproducts in bulk DNA between Scid and wildtype cells, as measured by cellular survival and unscheduled DNA synthesis. However, deficient repair was evident in genes, where Scid cells had 25-50% less repair in the c-myc and dihydrofolate reductase genes. After γ-irradiation, Scid fibroblasts had 20-35% less repair of DNA breaks in immunoglobulin κ and heavy constant genes than wildtype cells. The data suggest that intact DNA-PK enzyme is needed for the efficient operation of cellular repair of pyrimidine dimers and single-strand breaks in genes, as well as in its established role in rejoining double-strand breaks.

Original languageEnglish (US)
Pages (from-to)878-882
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume264
Issue number3
DOIs
StatePublished - Nov 2 1999

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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