TY - JOUR
T1 - Leptin increases small intestinal response to cholecystokinin in leptin-deficient obese mice
AU - Kiely, James M.
AU - Graewin, Shannon J.
AU - Pitt, Henry A.
AU - Swartz-Basile, Deborah A.
PY - 2005/3
Y1 - 2005/3
N2 - Introduction. Leptin receptors are present in the jejunum, ileum, and vagal neurons. Leptin increases duodenal secretion of cholecystokinin (CCK) and acts with CCK on vagal mechanoreceptors in the regulation of small intestinal motility. We have demonstrated that leptin-deficient (Lepob) obese mice have increased jejunal and normal ileal responses to CCK. Therefore, we hypothesized that leptin administration alters small intestinal motility observed in leptin-deficient obese mice. Materials and methods. Twelve-week-old female leptin-deficient (Lepob) obese mice received either saline (n = 12) or 5 μg/g leptin ip (n = 12) injections daily. After 4 weeks, jejunal and ileal segments were harvested, mounted in an organ bath, and reacted with acetylcholine (ACh, 10-5M) and CCK (10-8,-7,-6M). Data were expressed as N/cm2 and compared by ANOVA and Student's t test. Results. The average body weights in the leptin-treated group were significantly decreased compared to those of the saline-treated group (34 versus 49 g, P < 0.01). Jejunal responses to ACh within each group were significantly decreased (P < 0.05) when compared to ileal responses. No significant differences in responses to ACh were observed between groups. Jejunal responses to 10 -7,-6M CCK in the leptin-treated group were significantly greater than those in the saline-treated group. Ileal responses in the leptin group were similarly increased at all CCK concentrations. Conclusions. These data suggest that daily leptin administration for 4 weeks in leptin-deficient (Lep ob) obese mice increases jejunal and ileal responses to CCK and does not alter responses to ACh. Therefore, we conclude that regulation of small intestinal motility may be influenced by synergistic action of cholecystokinin and leptin.
AB - Introduction. Leptin receptors are present in the jejunum, ileum, and vagal neurons. Leptin increases duodenal secretion of cholecystokinin (CCK) and acts with CCK on vagal mechanoreceptors in the regulation of small intestinal motility. We have demonstrated that leptin-deficient (Lepob) obese mice have increased jejunal and normal ileal responses to CCK. Therefore, we hypothesized that leptin administration alters small intestinal motility observed in leptin-deficient obese mice. Materials and methods. Twelve-week-old female leptin-deficient (Lepob) obese mice received either saline (n = 12) or 5 μg/g leptin ip (n = 12) injections daily. After 4 weeks, jejunal and ileal segments were harvested, mounted in an organ bath, and reacted with acetylcholine (ACh, 10-5M) and CCK (10-8,-7,-6M). Data were expressed as N/cm2 and compared by ANOVA and Student's t test. Results. The average body weights in the leptin-treated group were significantly decreased compared to those of the saline-treated group (34 versus 49 g, P < 0.01). Jejunal responses to ACh within each group were significantly decreased (P < 0.05) when compared to ileal responses. No significant differences in responses to ACh were observed between groups. Jejunal responses to 10 -7,-6M CCK in the leptin-treated group were significantly greater than those in the saline-treated group. Ileal responses in the leptin group were similarly increased at all CCK concentrations. Conclusions. These data suggest that daily leptin administration for 4 weeks in leptin-deficient (Lep ob) obese mice increases jejunal and ileal responses to CCK and does not alter responses to ACh. Therefore, we conclude that regulation of small intestinal motility may be influenced by synergistic action of cholecystokinin and leptin.
KW - Cholecystokinin
KW - Lep mice
KW - Leptin
KW - Obesity
KW - Small intestinal motility
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U2 - 10.1016/j.jss.2004.10.006
DO - 10.1016/j.jss.2004.10.006
M3 - Article
C2 - 15734493
AN - SCOPUS:13944276005
SN - 0022-4804
VL - 124
SP - 146
EP - 150
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -