Leptin attenuates respiratory complications associated with the obese phenotype

C. G. Tankersley, C. O'Donnell, M. J. Daood, J. F. Watchko, Wayne A Mitzner, Alan R Schwartz, Philip L Smith

Research output: Contribution to journalArticle

Abstract

A profile of respiratory complications has been associated with the onset and development of obesity in humans. Similar phenotypes have been routinely demonstrated in genetic animal models of obesity such as the ob mouse (C57BL/6J-Lep(ob)). The objective of the present study was to test the hypothesis that a constellation of respiratory complications are attenuated with leptin (i.e., protein product of the ob gene) replacement. Daily leptin administration during a 6-wk period was conducted to control body weight of mutant ob mice similar to genotypic control groups. During the treatment period, repeated baseline ventilatory measurements were assessed by using whole body plethysmography while quasistatic pressure-volume curves were performed to further explore the role of leptin in improving lung mechanics. Diaphragmatic myosin heavy chain (MHC) isoform phenotype was examined to determine proportional changes in MHC composition. In room air, breathing frequency and minute ventilation were significantly (P <0.01) different among ob treatment groups, suggesting that leptin opposed the development of a rapid breathing pattern observed in vehicle-treated ob mice. Quasistatic deflation curves indicated that the lung volume of leptin-treated ob mice was significantly (P <0.05) greater relative to vehicle-treated ob mice at airway pressures between 0 and 30 cmH2O. Diaphragm MHC composition of leptin-treated ob mice was restored significantly (P <0.05) to resemble the control phenotype. In this genetic mouse model of obesity, the results suggested that respiratory complications associated with the obese phenotype, including rapid breathing pattern at baseline, diminished lung compliance, and abnormal respiratory muscle adaptations, are attenuated with prolonged leptin treatment.

Original languageEnglish (US)
Pages (from-to)2261-2269
Number of pages9
JournalJournal of Applied Physiology
Volume85
Issue number6
StatePublished - Dec 1998

Fingerprint

Leptin
Phenotype
Myosin Heavy Chains
Respiration
Obesity
Genetic Models
Whole Body Plethysmography
Lung Compliance
Pressure
Lung
Respiratory Muscles
Diaphragm
Mechanics
Inbred C57BL Mouse
Ventilation
Protein Isoforms
Animal Models
Air
Body Weight
Control Groups

Keywords

  • Diaphragm myosin heavy chain composition
  • Genetic control of ventilation
  • Lung growth and development
  • Lung pressure-volume curves

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Tankersley, C. G., O'Donnell, C., Daood, M. J., Watchko, J. F., Mitzner, W. A., Schwartz, A. R., & Smith, P. L. (1998). Leptin attenuates respiratory complications associated with the obese phenotype. Journal of Applied Physiology, 85(6), 2261-2269.

Leptin attenuates respiratory complications associated with the obese phenotype. / Tankersley, C. G.; O'Donnell, C.; Daood, M. J.; Watchko, J. F.; Mitzner, Wayne A; Schwartz, Alan R; Smith, Philip L.

In: Journal of Applied Physiology, Vol. 85, No. 6, 12.1998, p. 2261-2269.

Research output: Contribution to journalArticle

Tankersley, CG, O'Donnell, C, Daood, MJ, Watchko, JF, Mitzner, WA, Schwartz, AR & Smith, PL 1998, 'Leptin attenuates respiratory complications associated with the obese phenotype', Journal of Applied Physiology, vol. 85, no. 6, pp. 2261-2269.
Tankersley CG, O'Donnell C, Daood MJ, Watchko JF, Mitzner WA, Schwartz AR et al. Leptin attenuates respiratory complications associated with the obese phenotype. Journal of Applied Physiology. 1998 Dec;85(6):2261-2269.
Tankersley, C. G. ; O'Donnell, C. ; Daood, M. J. ; Watchko, J. F. ; Mitzner, Wayne A ; Schwartz, Alan R ; Smith, Philip L. / Leptin attenuates respiratory complications associated with the obese phenotype. In: Journal of Applied Physiology. 1998 ; Vol. 85, No. 6. pp. 2261-2269.
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