Leishmanization: Use of an old method for evaluation of candidate vaccines against leishmaniasis

Ali Khamesipour; Yahya Dowlati; Ali Asilian; Reza Hashemi-Fesharki; Amir Javadi; Sassan Noazin; Farrokh Modabber

doi:10.1016/j.vaccine.2005.02.015

Leishmanization: Use of an old method for evaluation of candidate vaccines against leishmaniasis

Ali Khamesipour, Yahya Dowlati, Ali Asilian, Reza Hashemi-Fesharki, Amir Javadi, Sassan Noazin, Farrokh Modabber

Research output: Contribution to journal › Article › peer-review

146 Scopus citations

Abstract

To establish the safety and reproducibility of live challenge with Leishmania major, a single frozen stabilate was used in two open, single-arm leishmanization (LZ) trials. A total of 42 inoculations in 28 male adult volunteers were followed until complete healing. Lesions induced by LZ are as diverse as natural infection, but much milder. Total protection was seen in 100% (11/11) of recovered volunteers. LZ used as live challenge to test vaccine candidates reduces the required sample size, is cost effective for surrogate markers studies and will induce protection in the participants who are not protected by candidate vaccines.

Original language	English (US)
Pages (from-to)	3642-3648
Number of pages	7
Journal	Vaccine
Volume	23
Issue number	28
DOIs	https://doi.org/10.1016/j.vaccine.2005.02.015
State	Published - May 25 2005
Externally published	Yes

Keywords

Iran
Leishmania
Leishmanization
Live challenge
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2005.02.015

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title = "Leishmanization: Use of an old method for evaluation of candidate vaccines against leishmaniasis",

abstract = "To establish the safety and reproducibility of live challenge with Leishmania major, a single frozen stabilate was used in two open, single-arm leishmanization (LZ) trials. A total of 42 inoculations in 28 male adult volunteers were followed until complete healing. Lesions induced by LZ are as diverse as natural infection, but much milder. Total protection was seen in 100% (11/11) of recovered volunteers. LZ used as live challenge to test vaccine candidates reduces the required sample size, is cost effective for surrogate markers studies and will induce protection in the participants who are not protected by candidate vaccines.",

keywords = "Iran, Leishmania, Leishmanization, Live challenge, Vaccine",

author = "Ali Khamesipour and Yahya Dowlati and Ali Asilian and Reza Hashemi-Fesharki and Amir Javadi and Sassan Noazin and Farrokh Modabber",

note = "Funding Information: The authors would like to thank Drs. R. Badaro, R. Berquist, E.A. Khalil, J. Mauel, D. Sacks, P. Smith, F. Zicker, J. Karbwang, and L. Moulton for visiting the site and checking lesions and M. Levine for encouragement. This project was financially supported by Under Secretary for Research, Ministry of Health and Medical Education, Iran. The UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) supported the follow up studies after leishmanization.",

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doi = "10.1016/j.vaccine.2005.02.015",

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AU - Khamesipour, Ali

AU - Dowlati, Yahya

AU - Asilian, Ali

AU - Hashemi-Fesharki, Reza

AU - Javadi, Amir

AU - Noazin, Sassan

AU - Modabber, Farrokh

N1 - Funding Information: The authors would like to thank Drs. R. Badaro, R. Berquist, E.A. Khalil, J. Mauel, D. Sacks, P. Smith, F. Zicker, J. Karbwang, and L. Moulton for visiting the site and checking lesions and M. Levine for encouragement. This project was financially supported by Under Secretary for Research, Ministry of Health and Medical Education, Iran. The UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) supported the follow up studies after leishmanization.

PY - 2005/5/25

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N2 - To establish the safety and reproducibility of live challenge with Leishmania major, a single frozen stabilate was used in two open, single-arm leishmanization (LZ) trials. A total of 42 inoculations in 28 male adult volunteers were followed until complete healing. Lesions induced by LZ are as diverse as natural infection, but much milder. Total protection was seen in 100% (11/11) of recovered volunteers. LZ used as live challenge to test vaccine candidates reduces the required sample size, is cost effective for surrogate markers studies and will induce protection in the participants who are not protected by candidate vaccines.

AB - To establish the safety and reproducibility of live challenge with Leishmania major, a single frozen stabilate was used in two open, single-arm leishmanization (LZ) trials. A total of 42 inoculations in 28 male adult volunteers were followed until complete healing. Lesions induced by LZ are as diverse as natural infection, but much milder. Total protection was seen in 100% (11/11) of recovered volunteers. LZ used as live challenge to test vaccine candidates reduces the required sample size, is cost effective for surrogate markers studies and will induce protection in the participants who are not protected by candidate vaccines.

KW - Iran

KW - Leishmania

KW - Leishmanization

KW - Live challenge

KW - Vaccine

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Leishmanization: Use of an old method for evaluation of candidate vaccines against leishmaniasis

Abstract

Keywords

ASJC Scopus subject areas

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