Leg metabolism of the amino acids and ammonia in patients with chronic renal failure

G. Deferrari, G. Garibotto, C. Robaudo, A. Canepa, Serena M Bagnasco, A. Tizianello

Research output: Contribution to journalArticle

Abstract

1. Leg metabolism of amino acids and ammonia in the postabsorptive state was evaluated in 10 patients with chronic renal failure (CRF) and in 10 patients with normal renal function (controls) by measuring the arterial-femoral venous (A-FV) differences for free amino acids and ammonia. 2. Total amino acid release from the leg and alanine and glutamine release, which accounts for the greatest amount of the total amino acid release, are similar in patients and controls. Total amino acid uptake from the arterial blood and glutamate uptake, which is the amino acid extracted at the highest rate, are comparable in both groups. Taken together these data, in addition to the similarity of A-FV differences for proteolytic markers, namely tyrosine, phenylalanine and histidine, suggest that protein breakdown in peripheral tissues is not increased in patients with CRF. 3. In CRF selective metabolic abnormalities for some amino acids are evident. Whilst only the A-FV differences for valine, leucine, and isoleucine are decreased, additional alterations are observed by relating the A-FV difference for each amino acid to that of proteolytic markers. Such a procedure demonstrates that in CRF histidine release relative to that of proteolytic markers is reduced, whereas proline and arginine release is increased. 4. In CRF the reduced release of some amino acids, mainly branched amino acids, by the leg probably affects the pattern of circulating amino acids. 5. Finally, both in patients and in controls a significant uptake of ammonia is observed; the ammonia uptake is related to arterial levels of this metabolite, confirming the role of peripheral tissues in removing ammonia from circulation.

Original languageEnglish (US)
Pages (from-to)143-151
Number of pages9
JournalClinical Science
Volume69
Issue number2
StatePublished - 1985
Externally publishedYes

Fingerprint

Ammonia
Chronic Kidney Failure
Leg
Amino Acids
Thigh
Histidine
Isoleucine
Valine
Glutamine
Phenylalanine
Proline
Leucine
Alanine
Tyrosine
Arginine
Glutamic Acid
Kidney

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Deferrari, G., Garibotto, G., Robaudo, C., Canepa, A., Bagnasco, S. M., & Tizianello, A. (1985). Leg metabolism of the amino acids and ammonia in patients with chronic renal failure. Clinical Science, 69(2), 143-151.

Leg metabolism of the amino acids and ammonia in patients with chronic renal failure. / Deferrari, G.; Garibotto, G.; Robaudo, C.; Canepa, A.; Bagnasco, Serena M; Tizianello, A.

In: Clinical Science, Vol. 69, No. 2, 1985, p. 143-151.

Research output: Contribution to journalArticle

Deferrari, G, Garibotto, G, Robaudo, C, Canepa, A, Bagnasco, SM & Tizianello, A 1985, 'Leg metabolism of the amino acids and ammonia in patients with chronic renal failure', Clinical Science, vol. 69, no. 2, pp. 143-151.
Deferrari G, Garibotto G, Robaudo C, Canepa A, Bagnasco SM, Tizianello A. Leg metabolism of the amino acids and ammonia in patients with chronic renal failure. Clinical Science. 1985;69(2):143-151.
Deferrari, G. ; Garibotto, G. ; Robaudo, C. ; Canepa, A. ; Bagnasco, Serena M ; Tizianello, A. / Leg metabolism of the amino acids and ammonia in patients with chronic renal failure. In: Clinical Science. 1985 ; Vol. 69, No. 2. pp. 143-151.
@article{ea432a298d384dc2b8ca848dd41fc7f9,
title = "Leg metabolism of the amino acids and ammonia in patients with chronic renal failure",
abstract = "1. Leg metabolism of amino acids and ammonia in the postabsorptive state was evaluated in 10 patients with chronic renal failure (CRF) and in 10 patients with normal renal function (controls) by measuring the arterial-femoral venous (A-FV) differences for free amino acids and ammonia. 2. Total amino acid release from the leg and alanine and glutamine release, which accounts for the greatest amount of the total amino acid release, are similar in patients and controls. Total amino acid uptake from the arterial blood and glutamate uptake, which is the amino acid extracted at the highest rate, are comparable in both groups. Taken together these data, in addition to the similarity of A-FV differences for proteolytic markers, namely tyrosine, phenylalanine and histidine, suggest that protein breakdown in peripheral tissues is not increased in patients with CRF. 3. In CRF selective metabolic abnormalities for some amino acids are evident. Whilst only the A-FV differences for valine, leucine, and isoleucine are decreased, additional alterations are observed by relating the A-FV difference for each amino acid to that of proteolytic markers. Such a procedure demonstrates that in CRF histidine release relative to that of proteolytic markers is reduced, whereas proline and arginine release is increased. 4. In CRF the reduced release of some amino acids, mainly branched amino acids, by the leg probably affects the pattern of circulating amino acids. 5. Finally, both in patients and in controls a significant uptake of ammonia is observed; the ammonia uptake is related to arterial levels of this metabolite, confirming the role of peripheral tissues in removing ammonia from circulation.",
author = "G. Deferrari and G. Garibotto and C. Robaudo and A. Canepa and Bagnasco, {Serena M} and A. Tizianello",
year = "1985",
language = "English (US)",
volume = "69",
pages = "143--151",
journal = "Clinical Science",
issn = "0143-5221",
publisher = "Portland Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Leg metabolism of the amino acids and ammonia in patients with chronic renal failure

AU - Deferrari, G.

AU - Garibotto, G.

AU - Robaudo, C.

AU - Canepa, A.

AU - Bagnasco, Serena M

AU - Tizianello, A.

PY - 1985

Y1 - 1985

N2 - 1. Leg metabolism of amino acids and ammonia in the postabsorptive state was evaluated in 10 patients with chronic renal failure (CRF) and in 10 patients with normal renal function (controls) by measuring the arterial-femoral venous (A-FV) differences for free amino acids and ammonia. 2. Total amino acid release from the leg and alanine and glutamine release, which accounts for the greatest amount of the total amino acid release, are similar in patients and controls. Total amino acid uptake from the arterial blood and glutamate uptake, which is the amino acid extracted at the highest rate, are comparable in both groups. Taken together these data, in addition to the similarity of A-FV differences for proteolytic markers, namely tyrosine, phenylalanine and histidine, suggest that protein breakdown in peripheral tissues is not increased in patients with CRF. 3. In CRF selective metabolic abnormalities for some amino acids are evident. Whilst only the A-FV differences for valine, leucine, and isoleucine are decreased, additional alterations are observed by relating the A-FV difference for each amino acid to that of proteolytic markers. Such a procedure demonstrates that in CRF histidine release relative to that of proteolytic markers is reduced, whereas proline and arginine release is increased. 4. In CRF the reduced release of some amino acids, mainly branched amino acids, by the leg probably affects the pattern of circulating amino acids. 5. Finally, both in patients and in controls a significant uptake of ammonia is observed; the ammonia uptake is related to arterial levels of this metabolite, confirming the role of peripheral tissues in removing ammonia from circulation.

AB - 1. Leg metabolism of amino acids and ammonia in the postabsorptive state was evaluated in 10 patients with chronic renal failure (CRF) and in 10 patients with normal renal function (controls) by measuring the arterial-femoral venous (A-FV) differences for free amino acids and ammonia. 2. Total amino acid release from the leg and alanine and glutamine release, which accounts for the greatest amount of the total amino acid release, are similar in patients and controls. Total amino acid uptake from the arterial blood and glutamate uptake, which is the amino acid extracted at the highest rate, are comparable in both groups. Taken together these data, in addition to the similarity of A-FV differences for proteolytic markers, namely tyrosine, phenylalanine and histidine, suggest that protein breakdown in peripheral tissues is not increased in patients with CRF. 3. In CRF selective metabolic abnormalities for some amino acids are evident. Whilst only the A-FV differences for valine, leucine, and isoleucine are decreased, additional alterations are observed by relating the A-FV difference for each amino acid to that of proteolytic markers. Such a procedure demonstrates that in CRF histidine release relative to that of proteolytic markers is reduced, whereas proline and arginine release is increased. 4. In CRF the reduced release of some amino acids, mainly branched amino acids, by the leg probably affects the pattern of circulating amino acids. 5. Finally, both in patients and in controls a significant uptake of ammonia is observed; the ammonia uptake is related to arterial levels of this metabolite, confirming the role of peripheral tissues in removing ammonia from circulation.

UR - http://www.scopus.com/inward/record.url?scp=0021867935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021867935&partnerID=8YFLogxK

M3 - Article

C2 - 4064563

AN - SCOPUS:0021867935

VL - 69

SP - 143

EP - 151

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

IS - 2

ER -