Lectin staining and Western blot data showing differential sialylation of nutrient-deprived cancer cells to sialic acid supplementation

Haitham A. Badr, Dina M.M. AlSadek, Mohit P. Mathew, Chen Zhong Li, Leyla B. Djansugurova, Kevin J. Yarema, Hafiz Ahmed

Research output: Contribution to journalArticle

Abstract

This report provides data that are specifically related to the differential sialylation of nutrient deprived breast cancer cells to sialic acid supplementation in support of the research article entitled, "Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation" [1]. Particularly, breast cancer cells, when supplemented with sialic acid under nutrient deprivation, display sialylated glycans at the cell surface, but non-malignant mammary cells show sialylated glycans intracellularly. The impact of sialic acid supplementation under nutrient deprivation was demonstrated by measuring levels of expression and sialylation of two markers, EGFR1 and MUC1. This Data in Brief article complements the main manuscript by providing detailed instructions and representative results for cell-level imaging and Western blot analyses of changes in sialylation during nutrient deprivation and sialic acid supplementation. These methods can be readily generalized for the study of many types of glycosylation and various glycoprotein markers through the appropriate selection of fluorescently-labeled lectins.

Original languageEnglish (US)
Pages (from-to)481-488
Number of pages8
JournalData in Brief
Volume5
DOIs
StatePublished - Dec 1 2015

Keywords

  • Aberrant glycosylation
  • Differential sialylation
  • Metabolic glycoengineering
  • Nutrient deprivation
  • Sialic acid biosynthesis

ASJC Scopus subject areas

  • General

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