Lectin-like attachment sites on murine pulmonary alveolar macrophages bind Aspergillus fumigatus conidia

V. L. Kan, J. E. Bennett

Research output: Contribution to journalArticlepeer-review

Abstract

Murine pulmonary alveolar macrophages bound Aspergillus fumigatus conidia in vitro at 4 C and 37 C in the absence of serum or opsonins. This attachment was dependent on calcium and was sensitive to mild trypsinization and paraformaldehyde pretreatment of the macrophage membrane. Chitotriose, N-acetylglucosamine, D-mannose, α-methyl-mannoside, and L-fucose, but not D-galactose, were effective inhibitors of conidial binding. This pattern of reduction of conidial binding was consistent with that for the mannosyl-fucosyl receptor. In addition, conidial binding may be mediated by another lectin on the macrophage membrane, one that recognizes chitin components, because N-acetylglucosamine and chitotriose exhibited greater inhibition than expected for the mannosyl-fucosyl lectin.

Original languageEnglish (US)
Pages (from-to)407-414
Number of pages8
JournalJournal of Infectious Diseases
Volume158
Issue number2
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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