Layer-by-layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery

Corey J. Bishop, Allen L. Liu, David S. Lee, Richard J. Murdock, Jordan J. Green

Research output: Contribution to journalArticle

Abstract

Nonviral gene delivery methods represent a potential safe and effective approach for treating myriad diseases. For many gene therapy applications, delivering multiple exogenous genes and controlling the time profile that these genes are expressed would be advantageous. Polymeric nonviral gene carriers are versatile and can be readily tailored for particular therapeutic applications, have the ability to carry multiple large genes within each particle, and can be more easily manufactured than viruses used for gene delivery. A layer-by-layer (LbL) theranostic-enabling nanoparticle was developed to incorporate two plasmid types which have differing expression time profiles. Temporally controlling the expression of exogenous DNA enables superior control over the microenvironment and could lead to better control over differentiation pathways and cell fate.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume104
Issue number3
DOIs
StatePublished - Mar 1 2016

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Keywords

  • cell engineering
  • gene delivery
  • gold nanoparticle
  • layer-by-layer
  • poly(beta-amino ester)

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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