Abstract
Using a rat model of stroke, we studied the effect of unilateral middle cerebral artery ligation on cerebrospinal fluid monoamine metabolites at different intervals over a 40-day postoperative period. Male Sprague-Dawley rats were divided into four groups: An unoperated control group (n = 9), a sham-operated group (n = 9), aright middle cerebral artery ligation group (n= 10), and a left middle cerebral artery ligation group (n= 10). One hundred microliters of cerebrospinal fluid were collected percutaneously from the ce rebellomedullary cistern just before and 5, 20, and 40 days after the surgical procedure. Monoamine metabolites — 3-methoxy-4-hydroxyphenylglycol (MHPG), 5-hydroxyindoleacetic acid (5-HIAA), and homovanillic add (HVA) - were measured using high-performance liquid chromatography. MHPG concentration in the right lesion group was significantly depleted from control levels 5, 20, and 40 days after surgery. No such depletion was observed in the left lesion rats. Concentration of 5-HIAA was relatively lower at Days 5 and 20 in the right lesion group than in the left lesion group. HVA concentration did not differ among the groups at any time. Our study has demonstrated a differential effect of unilateral Ischemia on cerebrospinal fluid neurochemistry in rats dependent on the cerebral hemisphere involved.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 472-475 |
| Number of pages | 4 |
| Journal | Stroke |
| Volume | 19 |
| Issue number | 4 |
| State | Published - 1988 |
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Keywords
- Catecholamines
- Cerebral infarction
- Cerebrospinal fluid
- Rats
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Clinical Neurology
- Advanced and Specialized Nursing
- Neuroscience(all)
Cite this
Lateralized effect of cerebral infarction on spinal fluid monoamine metabolite concentrations in rats. / Parikh, Rajesh M.; Justice, Alan; Moran, Timothy H; Robinson, Robert G.
In: Stroke, Vol. 19, No. 4, 1988, p. 472-475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lateralized effect of cerebral infarction on spinal fluid monoamine metabolite concentrations in rats
AU - Parikh, Rajesh M.
AU - Justice, Alan
AU - Moran, Timothy H
AU - Robinson, Robert G.
PY - 1988
Y1 - 1988
N2 - Using a rat model of stroke, we studied the effect of unilateral middle cerebral artery ligation on cerebrospinal fluid monoamine metabolites at different intervals over a 40-day postoperative period. Male Sprague-Dawley rats were divided into four groups: An unoperated control group (n = 9), a sham-operated group (n = 9), aright middle cerebral artery ligation group (n= 10), and a left middle cerebral artery ligation group (n= 10). One hundred microliters of cerebrospinal fluid were collected percutaneously from the ce rebellomedullary cistern just before and 5, 20, and 40 days after the surgical procedure. Monoamine metabolites — 3-methoxy-4-hydroxyphenylglycol (MHPG), 5-hydroxyindoleacetic acid (5-HIAA), and homovanillic add (HVA) - were measured using high-performance liquid chromatography. MHPG concentration in the right lesion group was significantly depleted from control levels 5, 20, and 40 days after surgery. No such depletion was observed in the left lesion rats. Concentration of 5-HIAA was relatively lower at Days 5 and 20 in the right lesion group than in the left lesion group. HVA concentration did not differ among the groups at any time. Our study has demonstrated a differential effect of unilateral Ischemia on cerebrospinal fluid neurochemistry in rats dependent on the cerebral hemisphere involved.
AB - Using a rat model of stroke, we studied the effect of unilateral middle cerebral artery ligation on cerebrospinal fluid monoamine metabolites at different intervals over a 40-day postoperative period. Male Sprague-Dawley rats were divided into four groups: An unoperated control group (n = 9), a sham-operated group (n = 9), aright middle cerebral artery ligation group (n= 10), and a left middle cerebral artery ligation group (n= 10). One hundred microliters of cerebrospinal fluid were collected percutaneously from the ce rebellomedullary cistern just before and 5, 20, and 40 days after the surgical procedure. Monoamine metabolites — 3-methoxy-4-hydroxyphenylglycol (MHPG), 5-hydroxyindoleacetic acid (5-HIAA), and homovanillic add (HVA) - were measured using high-performance liquid chromatography. MHPG concentration in the right lesion group was significantly depleted from control levels 5, 20, and 40 days after surgery. No such depletion was observed in the left lesion rats. Concentration of 5-HIAA was relatively lower at Days 5 and 20 in the right lesion group than in the left lesion group. HVA concentration did not differ among the groups at any time. Our study has demonstrated a differential effect of unilateral Ischemia on cerebrospinal fluid neurochemistry in rats dependent on the cerebral hemisphere involved.
KW - Catecholamines
KW - Cerebral infarction
KW - Cerebrospinal fluid
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=0023940671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023940671&partnerID=8YFLogxK
M3 - Article
C2 - 2452499
AN - SCOPUS:0023940671
VL - 19
SP - 472
EP - 475
JO - Stroke
JF - Stroke
SN - 0039-2499
IS - 4
ER -