Lateral gene transfer of O1 serogroup encoding genes of Vibrio cholerae

Sol González Fraga, Mariana Pichel, Norma Binsztein, Judith A. Johnson, John Glenn Morris, Oscar Colin Stine

Research output: Contribution to journalArticle

Abstract

In Gram-negative bacteria, the O-antigen-encoding genes may be transferred between lineages, although mechanisms are not fully understood. To assess possible lateral gene transfer (LGT), 21 Argentinean Vibrio cholerae O-group 1 (O1) isolates were examined using multilocus sequence typing (MLST) to determine the genetic relatedness of housekeeping genes and genes from the O1 gene cluster. MSLT analysis revealed that 4.4% of the nucleotides in the seven housekeeping loci were variable, with six distinct genetic lineages identified among O1 isolates. In contrast, MLST analysis of the eight loci from the O1 serogroup region revealed that 0.24% of the 4943 nucleotides were variable. A putative breakpoint was identified in the JUMPstart sequence. Nine conserved nucleotides differed by a single nucleotide from a DNA uptake signal sequence (USS) also found in Pastuerellaceae. Our data indicate that genes in the O1 biogenesis region are closely related even in distinct genetic lineages, indicative of LGT, with a putative DNA USS identified at the defined boundary for the DNA exchange.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalFEMS Microbiology Letters
Volume286
Issue number1
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Horizontal Gene Transfer
Vibrio cholerae
Multilocus Sequence Typing
Nucleotides
Protein Sorting Signals
Genes
DNA
Housekeeping
O Antigens
Essential Genes
Multigene Family
Gram-Negative Bacteria
Serogroup

Keywords

  • Lateral gene transfer
  • Multilocus sequence typing
  • Vibrio cholerae

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Fraga, S. G., Pichel, M., Binsztein, N., Johnson, J. A., Morris, J. G., & Colin Stine, O. (2008). Lateral gene transfer of O1 serogroup encoding genes of Vibrio cholerae. FEMS Microbiology Letters, 286(1), 32-38. https://doi.org/10.1111/j.1574-6968.2008.01251.x

Lateral gene transfer of O1 serogroup encoding genes of Vibrio cholerae. / Fraga, Sol González; Pichel, Mariana; Binsztein, Norma; Johnson, Judith A.; Morris, John Glenn; Colin Stine, Oscar.

In: FEMS Microbiology Letters, Vol. 286, No. 1, 09.2008, p. 32-38.

Research output: Contribution to journalArticle

Fraga, SG, Pichel, M, Binsztein, N, Johnson, JA, Morris, JG & Colin Stine, O 2008, 'Lateral gene transfer of O1 serogroup encoding genes of Vibrio cholerae', FEMS Microbiology Letters, vol. 286, no. 1, pp. 32-38. https://doi.org/10.1111/j.1574-6968.2008.01251.x
Fraga SG, Pichel M, Binsztein N, Johnson JA, Morris JG, Colin Stine O. Lateral gene transfer of O1 serogroup encoding genes of Vibrio cholerae. FEMS Microbiology Letters. 2008 Sep;286(1):32-38. https://doi.org/10.1111/j.1574-6968.2008.01251.x
Fraga, Sol González ; Pichel, Mariana ; Binsztein, Norma ; Johnson, Judith A. ; Morris, John Glenn ; Colin Stine, Oscar. / Lateral gene transfer of O1 serogroup encoding genes of Vibrio cholerae. In: FEMS Microbiology Letters. 2008 ; Vol. 286, No. 1. pp. 32-38.
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