Lateral diffusion in plasma membrane of mouse egg is restricted after fertilisation

Martin Johnson, Michael A Edidin

Research output: Contribution to journalArticle

Abstract

Te functional properties of egg membranes change rapidly at fertilisation1. A physical basis for the earliest changes may be inferred from the observed rapid alterations in ion permeability and from the transient and prolonged changes of electrical potential2,3. Subsequently, new membrane is added to the egg surface during exocytosis of cortical granules, and some surface is selectively shed in polar and midbodies4,5. The physical changes occurring in the egg membrane, and the changed functional properties consequent upon them, may involve alterations of the mobility and association of lipids and proteins. Here, we present the results of studies on a general physical property, apparent lateral diffusion rate, of lipids and proteins in the membranes of unfertilised and fertilised mouse eggs. The single-cell photobleaching technique developed for measurement of diffusion in the surface membrane of cultured cells6-8 has been used to measure diffusion of the fluorescent dye 3,3-dioctadecylindocarbocyanine iodide (diI-C18) dissolved in surface membrane lipids and of fluorescent-labelled antibody fragment bound to a spectrum of surface membrane proteins. Our results indicate that diffusion of the lipid probe and of proteins in the membrane of unfertilised eggs occurs at about the maximum rate expected for these molecules. In fertilised eggs, both markers seem to diffuse at greatly reduced rates.

Original languageEnglish (US)
Pages (from-to)448-450
Number of pages3
JournalNature
Volume272
Issue number5652
DOIs
StatePublished - 1978
Externally publishedYes

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Fertilization
Ovum
Membrane Proteins
Cell Membrane
Membranes
Zygote
Lipids
Photobleaching
Immunoglobulin Fragments
Exocytosis
Iodides
Membrane Lipids
Fluorescent Dyes
Permeability
Ions
Proteins

ASJC Scopus subject areas

  • General

Cite this

Lateral diffusion in plasma membrane of mouse egg is restricted after fertilisation. / Johnson, Martin; Edidin, Michael A.

In: Nature, Vol. 272, No. 5652, 1978, p. 448-450.

Research output: Contribution to journalArticle

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