TY - JOUR
T1 - Laser-induced fluorescence during photorefractive keratectomy
T2 - A method for controlling epithelial removal
AU - Phillips, A. F.
AU - McDonnell, P. J.
N1 - Funding Information:
From the Doheny Eye Institute and the Department of Ophthalmology, University of Southern California School of Medicine, Los Angeles, California. This study was supported in part by a grant from the Autry Foundation, Los Angeles, California (Dr. McDonnell); National Eye Institute/National Institutes of Health core grant EY03040; and an unrestricted grant from Research to Prevent Blindness, Inc, New York, New York.
PY - 1997
Y1 - 1997
N2 - PURPOSE: Laser-induced fluorescence is generated during ablation of corneal tissue with the argon-fluoride 193-nm excimer laser. To investigate possible changes in laser-induced fluorescence spectra emitted during the transition between epithelium and stroma, we developed a system using an intensified charge-coupled device to achieve fast per-pulse temporal resolution of laser-induced fluorescence. METHODS: Freshly enucleated human cadaver eyes were subjected to 193-nm excimer laser keratectomy. During the procedure, laser-induced fluorescence was measured using an intensified charge-coupled device. Changes in laser-induced fluorescence were detected and used to control epithelial removal. Depth of ablation was determined histologically. RESULTS: The 193-nm excimer laser pulses induced both visible and ultraviolet fluorescence from corneal epithelium and stroma. In each layer two peaks predominated, one at 405 nm and the other at 346 nm. There was a rapid threefold reduction in the 346-nm ultraviolet peak at the transition from epithelium to stroma. CONCLUSIONS: By monitoring changes in laser-induced fluorescence at the epithelial-stromal interface, the clinician may be able to control corneal epithelial removal more precisely and reproducibly before performing photorefractive ablation.
AB - PURPOSE: Laser-induced fluorescence is generated during ablation of corneal tissue with the argon-fluoride 193-nm excimer laser. To investigate possible changes in laser-induced fluorescence spectra emitted during the transition between epithelium and stroma, we developed a system using an intensified charge-coupled device to achieve fast per-pulse temporal resolution of laser-induced fluorescence. METHODS: Freshly enucleated human cadaver eyes were subjected to 193-nm excimer laser keratectomy. During the procedure, laser-induced fluorescence was measured using an intensified charge-coupled device. Changes in laser-induced fluorescence were detected and used to control epithelial removal. Depth of ablation was determined histologically. RESULTS: The 193-nm excimer laser pulses induced both visible and ultraviolet fluorescence from corneal epithelium and stroma. In each layer two peaks predominated, one at 405 nm and the other at 346 nm. There was a rapid threefold reduction in the 346-nm ultraviolet peak at the transition from epithelium to stroma. CONCLUSIONS: By monitoring changes in laser-induced fluorescence at the epithelial-stromal interface, the clinician may be able to control corneal epithelial removal more precisely and reproducibly before performing photorefractive ablation.
UR - http://www.scopus.com/inward/record.url?scp=0031057432&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031057432&partnerID=8YFLogxK
U2 - 10.1016/S0002-9394(14)70990-0
DO - 10.1016/S0002-9394(14)70990-0
M3 - Article
C2 - 9186095
AN - SCOPUS:0031057432
SN - 0002-9394
VL - 123
SP - 42
EP - 47
JO - American journal of ophthalmology
JF - American journal of ophthalmology
IS - 1
ER -